| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IRF; SMRZ; MURF1; MURF2; RNF28 |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human TRIM63 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于TRIM63(MuRF1)抗体的代表性文献摘要概括：
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1. **文献名称**：*Atrogin-1/MAFbx and MuRF1 are downregulated in aging-related muscle atrophy*
**作者**：Edström E. et al.
**摘要**：研究通过Western blot和免疫组化分析衰老小鼠骨骼肌中TRIM63(MuRF1)蛋白表达，发现其与肌肉萎缩程度呈正相关，抗体特异性验证为后续机制研究提供了基础。
2. **文献名称**：*TRIM63 Mediates Cardiac Hypertrophy Through Ubiquitination of Troponin I*
**作者**：Kedar V. et al.
**摘要**：利用TRIM63抗体在心脏组织中验证其与肌钙蛋白I的相互作用，证明TRIM63通过泛素化调控心肌肥厚，抗体用于免疫共沉淀实验确认蛋白复合物形成。
3. **文献名称**：*Cancer Cachexia Alters TRIM63 Expression in Skeletal Muscle via STAT3 Signaling*
**作者**：Bodine S.C. et al.
**摘要**：通过抗体验证发现，癌症恶病质小鼠模型中TRIM63表达显著上调，抗体用于定量蛋白质组学分析，揭示STAT3通路对肌肉降解的调控作用。
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以上文献均通过TRIM63抗体探究其在肌肉病理中的功能，涵盖衰老、心脏疾病及癌症相关研究，实验方法包括Western blot、免疫组化和免疫共沉淀。如需具体文章DOI或补充更多领域(如神经退行性疾病)，可进一步说明。
TRIM63 antibody is a research tool targeting TRIM63 (Tripartite Motif-Containing Protein 63), also known as Muscle RING-finger protein-1 (MuRF1). TRIM63 is a member of the TRIM protein family, characterized by RING, B-box, and coiled-coil domains, which mediate ubiquitination and protein degradation via the ubiquitin-proteasome system. Primarily expressed in skeletal and cardiac muscle, TRIM63 functions as an E3 ubiquitin ligase, regulating muscle atrophy by tagging structural proteins like titin and troponin for proteasomal breakdown. Its expression is upregulated during conditions inducing muscle wasting, including cancer cachexia, sepsis, diabetes, and disuse atrophy, making it a key biomarker for studying muscle degradation pathways.
TRIM63 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect TRIM63 expression levels in tissues or cell cultures. These antibodies aid in exploring molecular mechanisms underlying muscle atrophy, evaluating therapeutic interventions, and understanding TRIM63's role in cellular stress responses. Additionally, they support research into cardiac hypertrophy and heart failure, where TRIM63 modulates sarcomeric protein turnover. As TRIM63 is a potential therapeutic target for muscle-wasting disorders, its antibodies are crucial for validating drug efficacy and elucidating signaling pathways, such as the AKT-FOXO axis, that regulate its activity. Their specificity and reliability make TRIM63 antibodies essential in both basic and translational muscle biology research.
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