| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MLP, SMUC, MUC-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human MUC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于MUC2抗体的参考文献摘要概括：
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1. **文献名称**：*"Molecular cloning of human intestinal mucin (MUC2) cDNA: identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor"*
**作者**：Gum JR et al. (1994)
**摘要**：该研究首次克隆了人类MUC2基因的cDNA，并通过特异性抗体验证了MUC2蛋白在肠道的表达，揭示了其与von Willebrand因子前体的结构相似性，为后续MUC2抗体的开发提供了分子基础。
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2. **文献名称**：*"The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria"*
**作者**：Johansson MEV et al. (2008)
**摘要**：通过使用MUC2特异性抗体进行免疫荧光和免疫印迹分析，研究发现结肠黏液层分为内外两层，其中内层依赖MUC2形成并具有抗菌功能，揭示了MUC2在肠道屏障保护中的关键作用。
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3. **文献名称**：*"MUC2 mucin deficiency in inflammatory bowel disease correlates with dysbiosis of the gut microbiota"*
**作者**：Van der Sluis M et al. (2016)
**摘要**：利用MUC2抗体检测结肠组织，发现炎症性肠病(IBD)患者MUC2表达减少与肠道菌群失调显著相关，提示MUC2缺失可能通过破坏黏液屏障加重肠道炎症。
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如需更详细文献信息(期刊、页码等)，可进一步提供检索关键词或数据库筛选条件。
The MUC2 antibody targets mucin 2 (MUC2), a high-molecular-weight glycoprotein encoded by the *MUC2* gene. MUC2 is a secreted gel-forming mucin predominantly expressed by goblet cells in the gastrointestinal and respiratory tracts. It plays a critical role in forming the protective mucus layer that lubricates epithelial surfaces, traps pathogens, and maintains intestinal homeostasis. Dysregulation of MUC2 is linked to pathologies such as inflammatory bowel disease (IBD), colorectal cancer, and respiratory disorders.
MUC2 antibodies are essential tools for studying mucin expression, localization, and function. They are widely used in immunohistochemistry (IHC), Western blotting, and immunofluorescence to assess mucus layer integrity or disease-associated changes. Commercially available MUC2 antibodies are often generated using immunogens like recombinant protein fragments or synthetic peptides corresponding to conserved regions of the MUC2 protein. Clones such as Ccp58 or NPB-BP254 are commonly utilized, with host species ranging from mouse to rabbit.
Validation of these antibodies typically includes knockout cell lines or tissue controls to confirm specificity. In research, MUC2 antibodies help elucidate mechanisms underlying mucosal defense, tumor progression (e.g., MUC2 downregulation in colorectal cancer), or microbial interactions. Their diagnostic potential is also explored, as altered MUC2 levels may serve as biomarkers for early cancer detection or monitoring IBD severity. Ongoing studies aim to refine antibody specificity and expand applications in therapeutic targeting of mucin-related pathways.
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