首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠cAMP反应元件结合蛋白(CREB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的CREB含量。CREB即环磷腺苷效应元件结合蛋白，在细胞生长、分化及代谢等多种生理过程中发挥关键作用。研究上，CREB主要通过与特定的DNA序列结合，调控下游基因表达来发挥功能，在神经可塑性、学习记忆、肿瘤发生等机制研究中备受关注。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于神经生物学、细胞信号转导机制研究、基因表达调控分析等领域，可助力探索CREB在生理或病理状态下的动态变化，或用于评估药物干预、环境刺激等外部因素对CREB表达水平的影响，为相关基础研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Creb1 ELISA Kit; Creb-1Cyclic AMP-responsive element-binding protein 1 ELISA Kit; CREB-1 ELISA Kit; cAMP-responsive element-binding protein 1 ELISA Kit
缩写：CREB
Uniprot No.：Q01147
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CREB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 87-103 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 86-105 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:800 | Average % | 90 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of mouse CREB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 83-105 | ||||
| EDTA plasma (n=4) | 95 | 87-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.7862.7232.7552.6191002.1282.0542.0911.955501.2641.2251.2451.109250.7720.8090.7910.65512.50.5480.5340.5410.4056.250.3510.3660.3590.2233.120.2390.2240.2320.09600.1370.1340.136 | ||||||
货期：3-5 working days
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