首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肉毒碱-棕榈酰基转移酶1，肝脏亚型(CPT1A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的CPT1A含量。CPT1A是一种重要的靶点。它在脂肪酸代谢中扮演关键角色，催化长链脂肪酸转运入线粒体进行β-氧化。对其研究发现，异常的CPT1A活性与多种疾病如代谢综合征、癌症等相关，探索其作用机制有望为相关疾病治疗提供新方向。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品可应用于基础医学研究中肝脏代谢功能评估、肥胖或糖尿病动物模型能量代谢通路分析、药物干预对脂肪酸氧化影响的体外实验等领域，为探索脂质代谢调控机制及相关疾病发病机理提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cpt1a; Cpt-1; Cpt1; Carnitine O-palmitoyltransferase 1, liver isoform; CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
缩写：CPT1A
Uniprot No.：P97742
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CPT1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-100 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 87-102 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse CPT1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 86-104 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.0062.0342.0201.8432001.3721.3851.3791.2021000.7350.7650.7500.573500.4310.4420.4370.260250.2860.2910.2890.11212.50.2370.2480.2430.0666.250.2150.2230.2190.04200.1740.1790.177 | ||||||
货期：3-5 working days
×
