| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ARB1; ARR1 |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARRB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ARRB1(β-arrestin-1)抗体的文献摘要示例(文献信息为模拟，仅供格式参考)：
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1. **文献名称**: *β-arrestin1 regulates oncogenic KRAS signaling in colorectal cancer*
**作者**: Smith A, et al.
**摘要**: 研究揭示了ARRB1在结直肠癌中通过调控KRAS信号通路促进肿瘤生长的机制，文中使用ARRB1抗体进行免疫共沉淀(Co-IP)验证其与KRAS的相互作用。
2. **文献名称**: *Structural basis of β-arrestin1 recruitment by G protein-coupled receptors*
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜技术解析ARRB1与GPCR结合的分子结构，实验中使用特异性ARRB1抗体进行Western blot验证蛋白表达，阐明受体激活后ARRB1的构象变化。
3. **文献名称**: *β-arrestin1 mediates macrophage polarization in inflammatory diseases*
**作者**: Wang Y, et al.
**摘要**: 探讨ARRB1在巨噬细胞M1/M2极化中的调控作用，通过ARRB1抗体进行免疫荧光染色，证实其在炎症模型中与TLR4通路的共定位。
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注：以上文献为示例，实际引用时需以真实发表的论文为准(可通过PubMed或Google Scholar搜索关键词“ARRB1 antibody”+“功能”查找具体研究)。
ARRB1 (β-arrestin-1) is a ubiquitously expressed intracellular scaffolding protein belonging to the arrestin family. It plays a critical role in regulating G protein-coupled receptor (GPCR) signaling by mediating receptor desensitization, internalization, and downstream signaling cascades. Beyond GPCR modulation, ARRB1 interacts with diverse signaling molecules (e.g., MAPK, AKT) and participates in non-GPCR pathways, including Wnt/β-catenin and NF-κB, influencing cell proliferation, migration, and apoptosis. Its dynamic subcellular shuttling between the cytoplasm and nucleus allows transcriptional regulation. Dysregulation of ARRB1 is implicated in cancer, neurological disorders, and metabolic diseases.
ARRB1 antibodies are essential tools for studying these processes. They enable detection of endogenous ARRB1 expression, post-translational modifications (e.g., phosphorylation), and protein-protein interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specific antibodies can distinguish ARRB1 from its homolog ARRB2 (β-arrestin-2) despite ~78% amino acid similarity. Validation includes testing in knockout cell lines or tissues to confirm specificity. Commercially available ARRB1 antibodies are typically raised against epitopes in the N- or C-terminal regions. Researchers use these antibodies to explore ARRB1's roles in disease mechanisms, drug response, and therapeutic targeting, particularly in contexts involving GPCR dysregulation or aberrant signal transduction.
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