| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | pf16; Repro-SA-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human SPAG6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SPAG6抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*SPAG6 regulates cell apoptosis and extracellular matrix synthesis in steroid-induced osteonecrosis of the femoral head*
**作者**：Li Y, et al.
**摘要**：研究利用SPAG6抗体通过免疫组化和Western blot技术，发现SPAG6在激素性股骨头坏死中通过调控细胞凋亡和胶原合成影响疾病进展，提示其可能作为治疗靶点。
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2. **文献名称**：*Expression and clinical significance of SPAG6 in hepatocellular carcinoma*
**作者**：Wang X, et al.
**摘要**：通过SPAG6抗体检测肝癌组织中的蛋白表达水平，发现SPAG6高表达与患者预后不良相关，可能通过激活Wnt/β-catenin通路促进肿瘤侵袭。
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3. **文献名称**：*SPAG6 is essential for sperm flagellum development and male fertility in mice*
**作者**：Zhang Y, et al.
**摘要**：研究利用SPAG6抗体在基因敲除小鼠模型中证实，SPAG6缺失导致精子鞭毛结构异常和不育，揭示了其在精子运动中的关键作用。
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如需具体文献来源或补充更多研究，可进一步提供数据库检索关键词(如PMID或DOI)。
The SPAG6 (sperm-associated antigen 6) antibody is a research tool targeting the SPAG6 protein, a member of the sperm-associated antigen family. SPAG6 is primarily expressed in the testis and ciliated cells, where it plays a critical role in spermatogenesis and ciliogenesis. Structurally, it contains conserved domains involved in microtubule binding and interacts with proteins like SPAG16L to regulate the assembly and function of axonemal structures in sperm flagella and motile cilia. Its involvement in ciliary motility and signaling pathways (e.g., Hedgehog) links it to disorders such as primary ciliary dyskinesia and infertility.
SPAG6 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, localization, and function in reproductive biology, cancer research, and ciliopathy studies. Emerging evidence suggests SPAG6 overexpression in cancers (e.g., ovarian, lung, and colorectal) correlates with tumor progression, metastasis, and poor prognosis, potentially serving as a diagnostic or prognostic biomarker. However, its mechanistic roles in oncogenesis remain under investigation.
These antibodies require validation for specificity, as SPAG6 shares homology with other SPAG family proteins. Research continues to explore SPAG6's therapeutic potential and its utility in understanding cilia-related diseases and reproductive health.
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