关键信息
-
基因名
CHAC1
-
简介
The CHAC1 protein cleaves glutathione, inducing apoptosis via the ATF4-ATF3-DDIT3/CHOP cascade. It acts as a negative regulator of the Notch signaling pathway, promoting neurogenesis by inhibiting Notch cleavage. CHAC1 Protein, Human is the recombinant human-derived CHAC1 protein, expressed by E. coli , with C-Strep.
- 应用
-
别名
CHAC1; Glutathione-specific gamma-glutamylcyclotransferase 1; Gamma-GCG 1; Blocks Notch protein; Botch; Cation transport regulator-like protein 1
-
种属
Human
-
表达系统
E. coli
-
标签
C-StrepⅡ
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9BUX1
-
表达区间
K2-V222
-
蛋白长度
Partial
-
分子量
27.2 kDa.
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CHAC1, or ChaC Glutathione Specific γ-Glutamylcyclotransferase 1, is a protein implicated in the cellular response to oxidative stress and is known for its role in glutathione metabolism. As a vital antioxidant, glutathione plays a key role in maintaining cellular redox homeostasis, and CHAC1 facilitates the degradation of glutathione, leading to an increase in reactive oxygen species (ROS) during stress conditions. This unique function positions CHAC1 as a critical player in various physiological and pathological processes, including inflammation, cancer progression, and neurodegenerative diseases. The recombinant production of CHAC1 has gained attention in research due to its potential therapeutic applications and the need to understand its functional dynamics in greater detail. Advances in recombinant protein technology have enabled the expression and purification of CHAC1, thereby allowing researchers to investigate its structure-function relationships, enzyme kinetics, and interaction with other cellular components. Furthermore, studying the recombinant CHAC1 protein provides insights into its regulatory mechanisms in the context of stress responses and how dysregulation may contribute to disease states. Overall, the exploration of CHAC1 as a recombinant protein not only advances our understanding of antioxidant defenses in cells but also opens avenues for the development of novel therapeutic strategies targeting oxidative stress-related disorders.
