首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠C反应蛋白(CRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的CRP含量。CRP即C反应蛋白，是一种急性时相反应蛋白。在炎症、感染、创伤等刺激下，肝脏合成CRP增加。其研究机制主要聚焦于它与炎症反应、心血管疾病等密切关联，可作为疾病诊断、病情监测和预后评估的重要标志物。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，该产品可广泛应用于科研领域，如炎症性疾病机制研究、实验动物模型炎症进程监测、药物或化合物抗炎效果评估等方向，为体外实验中CRP动态变化提供标准化检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Crp ELISA kit; Ptx1C-reactive protein ELISA kit
缩写：CRP
Uniprot No.：P48199
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-105 | |||||
回收率：
| The recovery of rat CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-97 | ||||
| EDTA plasma (n=4) | 96 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 20002.6642.7012.6832.541 10001.7011.7161.7091.567 5001.1731.1821.1781.036 2500.6040.5890.5970.455 1250.3980.3720.3850.243 62.50.3020.2880.2950.153 31.250.2340.2270.2310.089 00.1440.1400.142 | ||||||
货期：3-5 working days
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