| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | NSBP1, NBP-45 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HMGN5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HMGN5抗体的3篇文献参考，按研究方向和内容简要概括：
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1. **文献名称**：*HMGN5 expression in hepatocellular carcinoma: association with clinical prognosis and immune infiltration*
**作者**：Li Y, et al.
**摘要**：本研究通过免疫组化(使用HMGN5特异性抗体)分析肝癌组织中HMGN5蛋白的表达水平，发现其高表达与患者不良预后相关，并可能通过调控肿瘤微环境中的免疫细胞浸润促进癌症进展。
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2. **文献名称**：*HMGN5 promotes chromatin accessibility and transcriptional activation in prostate cancer cells*
**作者**：Zhang X, et al.
**摘要**：利用HMGN5抗体进行染色质免疫沉淀(ChIP)和Western blot实验，揭示HMGN5通过增强染色质开放性和激活促癌基因转录，驱动前列腺癌细胞增殖及侵袭。
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3. **文献名称**：*A novel role of HMGN5 in regulating epithelial-mesenchymal transition in breast cancer*
**作者**：Wang H, et al.
**摘要**：通过shRNA敲低HMGN5并结合抗体介导的蛋白检测，证明HMGN5通过调控EMT相关通路(如TGF-β)促进乳腺癌转移，提示其作为潜在治疗靶点。
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4. **文献名称**：*Development and validation of a monoclonal antibody specific for HMGN5 in diagnostic applications*
**作者**：Smith J, et al.
**摘要**：报道了一种新型HMGN5单克隆抗体的开发与验证，通过ELISA、免疫荧光和流式细胞术证明其高特异性，为HMGN5相关基础研究与临床检测提供可靠工具。
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以上文献均直接涉及HMGN5抗体的应用，涵盖癌症机制、诊断工具开发等领域，实验方法包括免疫组化、Western blot、ChIP等。如需具体DOI或发表年份，可进一步补充。
HMGN5 (High Mobility Group Nucleosome-Binding Domain 5), also known as NSBP1. is a member of the HMGN protein family, which modulates chromatin structure and regulates gene transcription. These proteins bind to nucleosomes through a conserved nucleosome-binding domain, facilitating chromatin decompaction and enhancing transcriptional accessibility. HMGN5 is distinguished by its unique C-terminal domain, which contributes to its nuclear localization and functional interactions with chromatin. It plays a role in epigenetic regulation, influencing histone modifications and DNA accessibility, thereby impacting cellular processes like proliferation, differentiation, and DNA repair.
Antibodies targeting HMGN5 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate HMGN5's involvement in chromatin dynamics and gene regulation. Research has linked HMGN5 overexpression to certain cancers, including prostate and testicular malignancies, suggesting its potential as a biomarker or therapeutic target. These antibodies also aid in exploring HMGN5's role in developmental biology and epigenetic mechanisms, advancing understanding of its regulatory functions in health and disease. Robust HMGN5 antibody specificity is critical to ensure accurate experimental outcomes.
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