首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠C反应蛋白(CRP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CRP含量。CRP即C反应蛋白，是一种急性时相反应蛋白。在炎症、感染、组织损伤等情况下其水平会迅速升高。研究机制方面，它可与病原体结合，激活补体系统，在免疫防御中发挥作用，也是临床常用的炎症和疾病活动监测指标。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，常用于评估机体免疫状态和炎症进程；特别适用于实验动物模型中炎症相关疾病研究、药物抗炎效果评价以及免疫调节机制探索等科研领域；可为心血管疾病、代谢综合征、感染性疾病等基础研究提供可靠的实验数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Crp ELISA Kit; Ptx1C-reactive protein ELISA Kit
缩写：CRP
Uniprot No.：P14847
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:40 | Average % | 103 | ||||
| Range % | 99-106 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:160 | Average % | 98 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of mouse CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.2062.1242.1651.9955001.6611.6551.6581.4882501.1381.1221.1300.9601250.7270.7210.7240.55462.50.4240.4350.4300.26031.20.2920.3050.2990.12915.60.2280.2340.2310.06100.1680.1720.170 | ||||||
本试剂盒所含材料：
An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
Biotin-labeled CRPantibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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