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产品描述：牛C反应蛋白(CRP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的CRP含量。CRP即C反应蛋白，是一种急性时相反应蛋白，在机体受到感染或组织损伤时，血浆中CRP水平会急剧上升。其可激活补体和加强吞噬细胞功能，在炎症反应中发挥重要作用。目前围绕它在疾病诊断、病情监测及药物靶点等方面的研究较多。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于兽医研究、畜牧业动物健康监测及炎症相关分子机制探索等科研领域，例如通过动态监测CRP水平评估牛群感染性疾病的发展趋势或疫苗免疫效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CRP
Uniprot No.：C4T8B4
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-88 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of bovine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-98 | ||||
| EDTA plasma (n=4) | 97 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 800.1980.1860.192 400.3150.3350.325 200.4860.4800.483 100.7410.7820.762 51.0271.1111.069 2.51.3301.4261.378 1.251.5461.5111.529 02.1452.2632.204 | ||||||
货期：3-5 working days
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