| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human NIT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NIT2抗体的3篇模拟参考文献示例(实际文献需通过学术数据库验证)：
1. **文献名称**：*NIT2 as a Novel Biomarker in Hepatocellular Carcinoma: Expression Analysis Using a Specific Polyclonal Antibody*
**作者**：Li X, Wang Y, Chen J
**摘要**：本研究通过制备兔源多克隆NIT2抗体，验证其在肝癌组织中的特异性表达，发现NIT2在肿瘤细胞中高表达且与患者预后相关，提示其作为潜在治疗靶点的可能性。
2. **文献名称**：*Characterization of NIT2 Antibody for Detecting Nitrilase Activity in Cellular Metabolism*
**作者**：Zhang R, Kumar A, Gupta S
**摘要**：利用单克隆NIT2抗体探究NIT2蛋白在尿素循环中的作用，通过免疫印迹和免疫荧光技术证实NIT2在肝细胞线粒体的定位，揭示其代谢调控功能。
3. **文献名称**：*Development of a High-Sensitivity NIT2 ELISA Kit for Neurological Disorder Diagnosis*
**作者**：Tanaka M, Suzuki K, Yamamoto T
**摘要**：基于新型NIT2抗体的ELISA检测方法被开发，用于分析阿尔茨海默病患者脑脊液样本，发现NIT2水平异常与疾病进展相关，为神经退行性疾病诊断提供新工具。
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**备注**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“NIT2 antibody”、“Nitrilase 2”或结合具体研究领域(如癌症、代谢疾病)筛选。
The NIT2 (Nitrilase-like Protein 2) antibody is a tool used to detect the NIT2 protein, a member of the nitrilase superfamily implicated in diverse cellular processes. NIT2 is evolutionarily conserved and ubiquitously expressed, particularly in the liver, kidney, and brain. Though its precise biochemical function remains under investigation, studies suggest it may act as a tumor suppressor by regulating cell proliferation and apoptosis. NIT2 is linked to metabolic pathways, potentially participating in amino acid metabolism or detoxification via nitrilase enzymatic activity.
In cancer research, NIT2 downregulation has been observed in hepatocellular carcinoma (HCC), often due to promoter hypermethylation, correlating with poor prognosis. The antibody aids in studying these expression patterns via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Beyond oncology, NIT2 is associated with neurological disorders; altered levels are noted in Alzheimer’s disease models, hinting at roles in neuronal survival.
The NIT2 antibody’s specificity is critical for distinguishing it from homologous proteins like NIT1. Validated in multiple species, it supports research into NIT2’s interaction partners and post-translational modifications. Its applications span molecular diagnostics, biomarker discovery, and mechanistic studies, highlighting its importance in unraveling NIT2’s pathophysiological roles and therapeutic potential.
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