首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠促肾上皮质激素释放激素(CRH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CRH含量。CRH（促肾上腺皮质素释放激素）是一种肽激素，参与应激反应，由CRH基因编码。它主要刺激垂体合成促肾上腺皮质激素。CRH在慢性疼痛和抑郁的神经环路中发挥重要作用，其调控机制涉及神经环路和突触传递，为相关疾病的治疗提供了新的靶点。试剂盒检测范围为1.25 ng/mL-20 ng/mL，该产品适用于科研领域中对大鼠应激模型、神经内分泌疾病机制的研究，以及通过检测外周或中枢组织中的 CRH 水平探究其生理病理调控机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Crh ELISA Kit; Corticoliberin ELISA Kit; Corticotropin-releasing factor ELISA Kit; CRF ELISA Kit; Corticotropin-releasing hormone ELISA Kit
缩写：CRH
Uniprot No.：P01143
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-20 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-93 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 97-105 | |||||
回收率：
| The recovery of rat CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.2440.2370.241 100.5090.5250.517 50.8540.9070.881 2.51.7571.7421.750 1.252.5712.4372.504 | ||||||
货期：3-5 working days
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