首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠促肾上腺皮质激素释放因子(CRF)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的CRH含量。促肾上腺皮质激素释放激素（CRH）是一种神经激素，在应激反应和神经内分泌调节中发挥关键作用。其研究机制主要围绕调节促肾上腺皮质激素（ACTH）释放，影响下丘脑-垂体-肾上腺轴（HPA轴）活性，还与情绪、焦虑等神经系统功能调节相关。试剂盒检测范围为2.5 pg/mL-40 pg/mL，适用于评估应激动物模型中的CRF动态变化、神经内分泌机制研究及药物干预效果验证等领域。为探究CRF在生理病理过程中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Crh ELISA kit; Gm1347Corticoliberin ELISA kit; Corticotropin-releasing factor ELISA kit; CRF ELISA kit; Corticotropin-releasing hormone ELISA kit
缩写：CRH
Uniprot No.：Q8CIT0
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：2.5 pg/mL-40 pg/mL
灵敏度：0.675 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 95-102 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of mouse CRF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-96 | ||||
| EDTA plasma (n=4) | 94 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average400.1680.1720.170200.3450.3490.347100.6780.6750.67751.2651.2691.2672.52.8512.8602.856 | ||||||
货期：3-5 working days
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