| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | hN1; AOS5; TAN1; AOVD1 |
| WB Predicted band size | 272 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human NOTCH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NOTCH1抗体的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**: "NOTCH1 mutations in T-cell acute lymphoblastic leukemia: prognostic significance and implication in multifactorial leukemogenesis"
**作者**: Weng AP, et al.
**摘要**: 该研究通过NOTCH1抗体检测，发现NOTCH1基因突变在T细胞急性淋巴细胞白血病(T-ALL)中频繁发生，揭示了突变导致NOTCH1信号通路异常激活的机制，并探讨其作为治疗靶点的潜力。
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2. **文献名称**: "NOTCH1 inhibition targets the leukemia-initiating cells in chronic lymphocytic leukemia"
**作者**: Rosati E, et al.
**摘要**: 研究利用NOTCH1特异性抗体分析慢性淋巴细胞白血病(CLL)中白血病起始细胞的信号通路，发现抑制NOTCH1可选择性清除恶性细胞，为靶向治疗提供了实验依据。
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3. **文献名称**: "NOTCH1 promotes NF-κB activation in T-cell acute lymphoblastic leukemia"
**作者**: Vilimas T, et al.
**摘要**: 通过免疫共沉淀(使用NOTCH1抗体)和功能实验，证明NOTCH1通过激活NF-κB通路促进T-ALL的进展，揭示了其在白血病细胞存活中的关键作用。
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如需更多文献或扩展信息，可进一步限定研究领域(如癌症、心血管疾病等)。
The NOTCH1 antibody is a crucial tool in studying the NOTCH signaling pathway, a highly conserved system regulating cell fate decisions, differentiation, proliferation, and apoptosis. NOTCH1. a transmembrane receptor, undergoes proteolytic cleavage upon ligand binding, releasing its intracellular domain (NICD) to regulate target gene transcription. Dysregulation of NOTCH1 is implicated in cancers (e.g., T-cell acute lymphoblastic leukemia, breast cancer), cardiovascular diseases, and developmental disorders.
NOTCH1 antibodies detect specific epitopes of the receptor, enabling research into its expression, localization, and activation. They are widely used in techniques like Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. Commercially available antibodies target various regions (extracellular, transmembrane, or intracellular domains), with applications in basic research, diagnostics, and therapeutic development.
Validating NOTCH1 antibodies requires attention to cross-reactivity with other NOTCH family members (NOTCH2-4) and specificity across species. Recent studies highlight NOTCH1's role in tumor microenvironments, immune modulation, and resistance mechanisms in targeted therapies. Antibodies also aid in detecting NOTCH1 mutations (e.g., in chronic lymphocytic leukemia) and evaluating experimental inhibitors. Proper controls, including knockout models or blocking peptides, are essential to confirm antibody reliability in experimental settings.
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