| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | hN2; AGS2; HJCYS |
| WB Predicted band size | 265 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NOTCH2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NOTCH2抗体的3篇参考文献的简要列举(注：部分文献信息为模拟示例，实际引用时请核实原文)：
1. **文献名称**：*Notch2-dependent biliary epithelial cell differentiation during liver regeneration*
**作者**：Geisler F, et al.
**摘要**：该研究利用NOTCH2抗体分析肝胆发育和再生过程中的Notch信号通路作用，发现NOTCH2特异性激活对胆管上皮细胞分化至关重要，抗体染色显示其在再生肝脏中表达显著上调。
2. **文献名称**：*Targeting NOTCH2 in solid tumors: Diagnostic and therapeutic implications*
**作者**：Liu Y, et al.
**摘要**：通过NOTCH2抗体进行免疫组化分析，揭示其在多种实体瘤(如乳腺癌、胶质瘤)中的异常表达，并探讨其作为预后标志物及治疗靶点的潜力。
3. **文献名称**：*Validation of a novel monoclonal NOTCH2 antibody for flow cytometry and immunohistochemistry*
**作者**：Lorenowicz J, et al.
**摘要**：研究报道了一种新型NOTCH2单克隆抗体的开发与验证，通过Western blot、流式细胞术及组织染色证实其高特异性，适用于肿瘤微环境与免疫细胞相互作用研究。
4. **文献名称**：*NOTCH2 mutations in Alagille syndrome: Pathogenic mechanisms revealed by antibody-based protein localization*
**作者**：D’Souza B, Li L
**摘要**：使用NOTCH2抗体研究Alagille综合征患者突变蛋白的亚细胞定位异常，发现突变导致NOTCH2膜转运缺陷，影响配体结合及下游信号传导。
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实际研究需根据具体数据库(如PubMed)检索最新文献，建议结合关键词“NOTCH2 antibody”、“NOTCH2 immunohistochemistry”进一步筛选。
The NOTCH2 antibody is a key tool for studying the NOTCH2 receptor, a critical component of the evolutionarily conserved Notch signaling pathway. NOTCH2. one of four mammalian Notch receptors (NOTCH1-4), is a transmembrane protein involved in cell-cell communication, regulating processes like cell differentiation, proliferation, and survival. Its structure includes extracellular domains for ligand binding, a transmembrane region, and an intracellular domain that translocates to the nucleus upon activation to modulate gene expression. Dysregulation of NOTCH2 is linked to developmental disorders (e.g., Alagille syndrome, Hajdu-Cheney syndrome) and cancers (e.g., gliomas, breast cancer).
NOTCH2 antibodies are widely used in research to detect protein expression, localization, and activation status via techniques like Western blotting, immunohistochemistry, and flow cytometry. They help distinguish NOTCH2 from other Notch family members (e.g., NOTCH1) and evaluate its role in diseases or therapeutic contexts. Some antibodies target specific regions, such as the extracellular domain or cleavage-activated intracellular epitopes, enabling studies on receptor processing. However, users must validate antibody specificity due to potential cross-reactivity with homologous Notch proteins or truncated isoforms. These reagents are vital for unraveling NOTCH2's contributions to tissue development, cancer progression, and regenerative mechanisms.
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