| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | DTD, QR1, DHQU, DIA4, NMOR1, NMORI |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human NQO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NQO1抗体的代表性文献摘要概括(基于公开研究数据，文献标题和作者为模拟示例)：
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1. **文献名称**: "NQO1 Antibody Validation for Immunohistochemical Analysis in Breast Cancer"
**作者**: Ross D. et al.
**摘要**: 本研究验证了兔源多克隆NQO1抗体在乳腺癌组织中的特异性，通过Western blot和免疫组化技术证明其能可靠检测肿瘤细胞中NQO1蛋白表达水平，并发现NQO1高表达与患者化疗耐药性相关。
2. **文献名称**: "Selective Detection of NQO1 in Lung Cancer Using a Novel Monoclonal Antibody"
**作者**: Siegel R., Hwang J.
**摘要**: 开发了一种新型小鼠单克隆NQO1抗体(克隆号4B7)，通过质谱分析确认其表位特异性。该抗体成功应用于肺癌细胞系和小鼠模型，显示NQO1在KRAS突变肿瘤中的表达显著上调。
3. **文献名称**: "NQO1 as a Biomarker in Colorectal Cancer: Antibody-Based Profiling and Clinical Correlations"
**作者**: Chen X. et al.
**摘要**: 利用商业化NQO1抗体(Abcam #ab34173)对结直肠癌组织进行检测，发现NQO1低表达患者对5-氟尿嘧啶治疗反应更佳，提示其作为化疗敏感性预测标志物的潜力。
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注：以上内容为基于领域知识的模拟摘要，实际文献需通过PubMed或Web of Science以"NQO1 antibody"、"NAD(P)H quinone dehydrogenase 1"等关键词检索获取。
The NAD(P)H:quinone oxidoreductase 1 (NQO1) antibody is a key tool for studying the NQO1 enzyme, a cytosolic flavoprotein involved in cellular antioxidant defense and detoxification. NQO1 catalyzes the two-electron reduction of quinones and other electrophilic compounds, preventing redox cycling and oxidative stress. It is regulated by the Nrf2-Keap1 pathway and plays dual roles in cancer biology: it detoxifies carcinogens in normal cells but may stabilize oncoproteins like p53 in tumors. Dysregulated NQO1 expression is linked to various cancers, neurodegenerative diseases, and metabolic disorders.
NQO1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and clinical correlations. Their specificity is critical due to structural similarities between NQO1 and related enzymes. Researchers often validate antibodies using knockout controls or recombinant proteins, particularly since the C609T polymorphism (rs1800566) generates a catalytically inactive NQO1 variant. Clone selection (e.g., A180. H6) and host species (mouse, rabbit) depend on experimental needs. These reagents support investigations into NQO1's roles in chemoresistance, biomarker potential, and therapeutic targeting. Proper validation ensures reliable detection of both wild-type and polymorphic variants across tissues.
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