| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | PIM |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PIM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CDH5(VE-cadherin)抗体的参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modify endothelial cell function*
**作者**：Corada M, et al.
**摘要**：该研究开发了针对VE-cadherin(CDH5)胞外结构域不同表位的单克隆抗体，发现部分抗体可破坏内皮细胞间粘附，抑制细胞连接的形成，揭示了VE-cadherin在维持内皮屏障完整性中的关键作用。
2. **文献名称**：*VEGF controls endothelial-cell permeability by promoting the β-arrestin-dependent endocytosis of VE-cadherin*
**作者**：Gavard J, Gutkind JS
**摘要**：研究证明VEGF通过β-arrestin依赖的内吞作用减少VE-cadherin(CDH5)在细胞连接处的表达，利用CDH5抗体追踪其内化过程，阐明了VEGF诱导血管通透性增加的分子机制。
3. **文献名称**：*VE-cadherin: the major endothelial adhesion molecule controlling cellular junctions and blood vessel formation*
**作者**：Vestweber D
**摘要**：综述总结了VE-cadherin(CDH5)在内皮细胞连接和血管生成中的核心功能，并讨论了其抗体在阻断实验中的应用，如在胚胎发育和肿瘤血管生成研究中的关键发现。
4. **文献名称**：*Endothelial destabilization by angiopoietin-2 via integrin β1 activation*
**作者**：Mayranpaa MI, et al.
**摘要**：研究发现Angiopoietin-2通过激活integrin β1导致VE-cadherin(CDH5)连接的破坏，利用CDH5抗体进行免疫染色，揭示了其在血管渗漏和炎症反应中的动态调控。
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这些文献展示了CDH5抗体在血管生物学研究中的多种应用，包括功能阻断、蛋白定位及机制探索。如需具体文献来源，建议通过PubMed或Google Scholar检索标题获取全文。
The CDH5 antibody is a crucial tool in biomedical research, targeting cadherin-5 (CDH5), also known as vascular endothelial cadherin (VE-cadherin). This calcium-dependent transmembrane glycoprotein is predominantly expressed in vascular endothelial cells, playing a pivotal role in maintaining endothelial cell-cell adhesion, vascular integrity, and angiogenesis. CDH5 regulates vascular permeability, leukocyte transmigration, and blood vessel formation through homophilic interactions and connections with cytoplasmic catenins.
CDH5 antibodies are widely used to study endothelial biology, tumor vasculature, and inflammatory diseases. They enable detection of CDH5 expression via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), helping researchers investigate vascular development, endothelial barrier function, and pathological conditions such as cancer metastasis, atherosclerosis, and sepsis. Many commercially available CDH5 antibodies are validated for specificity in human, mouse, or rat tissues, with monoclonal antibodies offering high reproducibility and polyclonal antibodies providing broader epitope recognition. Recent applications extend to flow cytometry and single-cell analysis, particularly in characterizing endothelial subtypes in complex tissues. Validation methods often include knockout (KO) controls or mass spectrometry to confirm target specificity. As endothelial dysfunction underlies numerous diseases, CDH5 antibodies remain essential for both basic research and therapeutic target validation in vascular biology.
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