首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅰ型胶原C端肽(CTX-Ⅰ)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTX-含量。CTX-Ⅰ是Ⅰ型胶原的降解产物，反映骨吸收活性。在严重烧伤或骨质疏松等病理状态下，CTX-Ⅰ水平升高，提示骨吸收增加。研究显示，CTX-Ⅰ可作为骨代谢异常的标志物，其机制与破骨细胞活性增加和成骨细胞功能受损有关。试剂盒检测范围为25 pg/mL-2000 pg/mL，骨代谢机制研究、骨质疏松动物模型评价、骨吸收抑制剂药效分析、骨相关疾病的基础研究和药物开发本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C-telopeptide of type 1 collagen,CTX-1,_x000D_C-telopeptide of type I collagen,CTX-I
缩写：CTX-
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-2000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of rat CTX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 86 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.3530.3540.354 7500.4680.4750.472 3750.6160.6050.611 1251.0110.9951.003 251.5121.5941.553 02.0132.1142.064 | ||||||
本试剂盒所含材料：
A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-human CTX-I antibody.
Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
Anti-rat CTX-I Antibody 1 x 6 ml -- Act as the detection antibody.
HRP-conjugated CTX-I 1 x 6 ml -- Bind to the CTX-I antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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