首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠组织蛋白酶S(CTSS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTSS含量。CTSS（组织蛋白酶S）是一种溶酶体半胱氨酸蛋白酶，参与抗原呈递和细胞外基质重塑。其在多种疾病如皮肤纤维瘤、肝纤维化中发挥作用，成为潜在的治疗靶点。研究显示，CTSS通过调节细胞外基质成分，影响疾病进程。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验中的蛋白表达水平监测、疾病机制研究及药物干预效果评价等科研领域，满足细胞实验、动物模型组织分析等多种研究需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctss ELISA Kit; Cathepsin S ELISA Kit; EC 3.4.22.27 ELISA Kit
缩写：CTSS
Uniprot No.：Q02765
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CTSS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of rat CTSS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 98-110 | ||||
| EDTA plasma (n=4) | 84 | 80-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5632.4572.5102.3955001.9972.0762.0371.9222501.3781.4041.3911.2761250.8770.8210.8490.73462.50.5670.5540.5610.44631.250.3580.3310.3450.23015.60.2270.2400.2340.11900.1130.1170.115 | ||||||
货期：3-5 working days
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