首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠组织蛋白酶S(CTSS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTSS含量。CTSS（组织蛋白酶S）是一种溶酶体半胱氨酸蛋白酶，参与细胞内蛋白质降解和抗原呈递，与免疫系统和多种疾病如神经退行性疾病、癌症和炎症反应密切相关。其研究机制主要涉及CTSS在细胞内外的表达、活性调控及其与底物和受体的相互作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒为研究CTSS在免疫调控、炎症通路或相关疾病机制中的功能提供高效工具，可应用于基础科研中的分子机制探索、药物干预效果评价或生物标志物筛查等方向。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctss ELISA kit; CatsCathepsin S ELISA kit; EC 3.4.22.27 ELISA kit
缩写：CTSS
Uniprot No.：O70370
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTSS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of mouse CTSS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-98 | ||||
| EDTA plasma (n=4) | 87 | 83-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8842.9522.9182.75010002.3452.4522.3992.2315001.6521.5491.6011.4332500.7290.7390.7340.5661250.4570.4520.4550.28762.50.2850.2970.2910.12331.250.2160.2190.2180.05000.1680.1670.168 | ||||||
货期：3-5 working days
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