首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠组织蛋白酶L1(CTSL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTSL1含量。CTSL1，即组织蛋白酶L1，是一种溶酶体半胱氨酸蛋白酶，在细胞内蛋白质分解代谢中起主要作用。其底物包括胶原蛋白和弹性蛋白，以及alpha-1蛋白酶抑制剂。CTSL1在多种病理过程中发挥作用，如肌病、心肌缺血和肾小管对蛋白尿的反应。研究显示，CTSL1还参与SARS-CoV-2刺突蛋白的切割，影响病毒进入细胞。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品适用于基础科研中CTSL1相关机制研究，如炎症信号通路解析、肿瘤微环境分析、细胞自噬调控等方向，尤其适合评估药物干预、基因敲除或过表达模型下的CTSL1动态变化，为体外实验及动物模型中蛋白功能研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctsl ELISA Kit; Ctsl1Cathepsin L1 ELISA Kit; EC 3.4.22.15 ELISA Kit; Cathepsin L ELISA Kit; Cyclic protein 2 ELISA Kit; CP-2 ELISA Kit; Major excreted protein ELISA Kit; MEP) [Cleaved into: Procathepsin L; Cathepsin L1 heavy chain; Cathepsin L1 light chain] ELISA Kit
缩写：CTSL1
Uniprot No.：P07154
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CTSL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 90-95 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:4 | Average % | 106 | |||||
| Range % | 103-109 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
回收率：
| The recovery of rat CTSL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 81-89 | |||||
| EDTA plasma (n=4) | 87 | 83-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected4001.9161.8431.8801.6982001.4521.4651.4591.2771000.9250.9320.9290.747500.5720.5810.5770.395250.4060.4360.4210.23912.50.3050.3000.3030.1216.250.2560.2490.2530.07100.1810.1830.182 | |||||||
货期：3-5 working days
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