| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ADH2; HEL-S-117 |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ADH1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于ADH1B抗体的**示例性参考文献**(内容为虚构，供参考格式。实际文献请通过PubMed、Google Scholar等平台查询)：
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1. **文献名称**: *"ADH1B as a Biomarker in Alcohol-Related Liver Disease: Immunohistochemical Analysis"*
**作者**: Smith J, et al. (2020)
**摘要**: 本研究利用抗ADH1B抗体，通过免疫组化技术检测酒精性肝病患者肝组织中ADH1B的表达水平，发现其表达与疾病严重程度呈负相关，提示ADH1B可能具有肝脏保护作用。
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2. **文献名称**: *"Development of a Monoclonal Antibody Specific to Human ADH1B for Diagnostic Applications"*
**作者**: Lee H, et al. (2018)
**摘要**: 报道了一种高特异性抗人ADH1B单克隆抗体的开发，验证其在ELISA和Western blot中的应用，证明其对ADH1B的选择性识别能力，为酒精代谢相关疾病的诊断提供工具。
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3. **文献名称**: *"ADH1B Genetic Variants and Protein Expression in Colorectal Cancer"*
**作者**: Garcia R, et al. (2019)
**摘要**: 通过抗ADH1B抗体分析结直肠癌组织中ADH1B蛋白表达，结合基因测序发现ADH1B rs1229984多态性与蛋白表达降低相关，可能影响肿瘤代谢通路。
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4. **文献名称**: *"Comparative Study of ADH1B Antibody Performance in Cross-Species Reactivity"*
**作者**: Chen T, et al. (2021)
**摘要**: 评估了不同物种(人、小鼠、大鼠)中ADH1B抗体的交叉反应性，发现某商业抗体对人源ADH1B特异性最佳，但对啮齿类动物存在非特异性结合，提示抗体选择需谨慎。
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**注意**：以上内容为模拟示例，真实文献需通过学术数据库检索(关键词如“ADH1B antibody”“ADH1B immunohistochemistry”)。建议结合具体研究场景筛选文献。
The ADH1B antibody is a crucial tool for studying alcohol dehydrogenase 1B (ADH1B), an enzyme encoded by the ADH1B gene in humans. ADH1B belongs to the alcohol dehydrogenase family, which catalyzes the oxidation of ethanol to acetaldehyde in the liver, playing a central role in alcohol metabolism. The ADH1B enzyme exhibits genetic polymorphisms, particularly the ADH1B*2 variant, which is associated with faster ethanol conversion and lower risk of alcohol dependence in certain populations.
ADH1B antibodies are widely used in research to detect and quantify ADH1B protein expression in tissues, notably in liver, stomach, and cancer studies. These antibodies enable techniques like Western blotting, immunohistochemistry (IHC), and ELISA to explore ADH1B's role in alcohol-related diseases, carcinogenesis, and metabolic disorders. For instance, reduced ADH1B expression has been linked to gastrointestinal cancers, while its overexpression may influence alcohol sensitivity.
Researchers also employ ADH1B antibodies to investigate tissue-specific expression patterns and genetic variations across ethnic groups. Both polyclonal and monoclonal versions are available, with selection depending on experimental needs for specificity and cross-reactivity. Validation parameters, including knockout controls and epitope mapping, ensure reliability in detecting this 40 kDa protein. Such studies contribute to understanding individual differences in alcohol metabolism and disease susceptibility.
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