首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠组织蛋白酶K(cath-K)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTSK含量。CTSK即组织蛋白酶K，是溶酶体半胱氨酸蛋白酶家族成员。它在破骨细胞骨吸收过程中发挥关键作用。研究发现其高表达与多种骨骼疾病相关，如骨质疏松等。目前围绕它开展了诸多研究，旨在开发针对性的药物来干预相关病理进程。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中骨吸收机制探索、破骨细胞功能评估或药物干预模型的疗效分析，为体外细胞实验、动物模型研究提供稳定的骨代谢相关分子检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctsk ELISA Kit; Cathepsin K ELISA Kit; EC 3.4.22.38 ELISA Kit
缩写：CTSK
Uniprot No.：P55097
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 100-112 | |||||
回收率：
| The recovery of mouse cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 90 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.8132.6872.7502.6025002.1982.2562.2272.0792501.5781.6671.6231.4751250.9410.9250.9330.78562.50.5730.5590.5660.41831.20.3210.3490.3350.18715.60.2460.2350.2410.09300.1510.1440.148 | ||||||
货期：3-5 working days
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