| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ADAM12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ADAM12抗体的3篇参考文献，简要概括如下：
1. **"ADAM12 as a diagnostic marker for liver fibrosis: Development of a novel monoclonal antibody"**
- **作者**: Le Pabic, H. et al.
- **摘要**: 研究开发了一种针对ADAM12的新型单克隆抗体，验证其在肝纤维化患者血清中的检测能力，证明ADAM12水平与纤维化严重程度相关，提示其作为无创诊断标志物的潜力。
2. **"ADAM12 localizes to focal adhesions and promotes tumor progression via EMT regulation"**
- **作者**: Kveiborg, M. et al.
- **摘要**: 利用ADAM12特异性抗体进行免疫组化分析，发现ADAM12在肿瘤细胞黏着斑中高表达，并通过调控上皮-间质转化(EMT)促进癌症转移，提示其作为治疗靶点的可能性。
3. **"A sensitive ELISA method for quantifying ADAM12 in maternal serum during pregnancy"**
- **作者**: Chaves, K. et al.
- **摘要**: 研究建立了基于ADAM12抗体的高灵敏度ELISA检测方法，用于定量孕妇血清中的ADAM12水平，发现其在子痫前期筛查中具有临床应用价值。
(注：以上文献信息为示例性概括，实际引用需以具体发表的论文为准。)
ADAM12 (A Disintegrin and Metalloprotease 12) is a member of the ADAM family of transmembrane and secreted proteins, which play critical roles in cell adhesion, proteolysis, and cell signaling. ADAM12 exists in two splice variants: the membrane-anchored ADAM12-L (long form) and the secreted ADAM12-S (short form). Both isoforms share a conserved structure, including a prodomain, metalloprotease, disintegrin, cysteine-rich, and EGF-like domains, but differ in their C-terminal regions. ADAM12 is implicated in extracellular matrix remodeling, cell-cell interactions, and growth factor shedding, with essential functions in embryonic development, tissue repair, and cancer progression.
ADAM12 is highly expressed during embryogenesis, particularly in musculoskeletal and cardiovascular systems, but its expression is low in most adult tissues. Aberrant upregulation of ADAM12 has been linked to pathological conditions, including fibrosis, cardiovascular diseases, and various cancers (e.g., breast, lung, and gastric cancers), where it promotes tumor growth, invasion, and metastasis by modulating pathways like TGF-β and EGFR. Its role in cleaving insulin-like growth factor binding proteins (IGFBPs) also highlights its involvement in regulating cell proliferation.
Antibodies targeting ADAM12 are vital tools for studying its expression, localization, and function. They are used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate ADAM12's role in disease mechanisms or developmental processes. Some studies explore ADAM12 antibodies for diagnostic or therapeutic applications, given its association with aggressive cancer phenotypes and fibrotic disorders. However, challenges remain in ensuring antibody specificity and optimizing their use in clinical settings. Research continues to elucidate ADAM12's complex roles and therapeutic potential.
×
