| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ADA2; ADA2A; KL04P; hADA2; TADA2L |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human TADA2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TADA2A抗体的3篇参考文献的简要概括：
1. **文献名称**：*TADA2A regulates histone acetylation and gene expression in the SAGA complex*
**作者**：Smith J, et al.
**摘要**：研究利用TADA2A抗体进行染色质免疫沉淀(ChIP)和免疫印迹(Western blot)，揭示了TADA2A在SAGA复合体中介导组蛋白H3乙酰化，调控靶基因转录的功能。
2. **文献名称**：*TADA2A mutations impair histone modifier activity and link to neurodevelopmental disorders*
**作者**：Lee S, et al.
**摘要**：通过TADA2A抗体检测患者细胞中的蛋白表达，发现突变导致TADA2A与组蛋白乙酰转移酶GCN5结合能力下降，提示其与神经发育异常的相关机制。
3. **文献名称**：*Proteomic analysis of TADA2A interactions in chromatin remodeling*
**作者**：Zhang R, et al.
**摘要**：采用TADA2A抗体进行免疫共沉淀(Co-IP)和质谱分析，鉴定了TADA2A与多个染色质重塑因子的相互作用，支持其在表观遗传调控网络中的枢纽作用。
(注：以上为模拟文献，实际文献需通过PubMed/Google Scholar检索关键词“TADA2A antibody”或“TADA2A function”获取。)
The TADA2A (Transcriptional Adaptor 2A) antibody is a tool used to study the TADA2A protein, a key component of the human SAGA (Spt-Ada-Gcn5 acetyltransferase) complex. TADA2A functions as a transcriptional coactivator, facilitating chromatin remodeling and histone acetylation via its interaction with histone acetyltransferases like GCN5. It plays a critical role in regulating gene expression, DNA repair, and cellular responses to stress. Dysregulation of TADA2A has been linked to developmental disorders, such as GAND syndrome (global developmental delay, absent or hypoplastic corpus callosum, and dysmorphic facies), and cancer progression.
TADA2A antibodies are typically developed in immunized hosts (e.g., rabbits or mice) using peptide antigens derived from conserved regions of the human TADA2A protein. These antibodies are validated for applications like Western blotting (WB), immunofluorescence (IF), and immunoprecipitation (IP). Specific clones or polyclonal preparations are commercially available from suppliers such as Santa Cruz Biotechnology, Abcam, and Cell Signaling Technology.
Researchers use TADA2A antibodies to investigate its role in chromatin dynamics, transcriptional regulation, and disease mechanisms. Validation often includes knockout cell lines to confirm specificity. Cross-reactivity with homologs in model organisms (e.g., mice) is sometimes assessed for translational studies. Proper controls, such as isotype-matched antibodies and antigen-blocking experiments, are essential to ensure reliable results.
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