首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠组织蛋白酶L1(CTSL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CTSL1含量。CTSL1，即组织蛋白酶L1，是一种溶酶体酸性半胱氨酸蛋白酶，在细胞内蛋白质分解代谢中起主要作用。其底物包括胶原蛋白和弹性蛋白，与肿瘤的发生、发展和转移有关，同时与炎症状态、动脉粥样硬化、肾病、糖尿病、骨病、病毒感染等疾病相关。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于基础研究中的细胞功能分析、疾病动物模型机制探索以及生物标志物筛选等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctsl ELISA kit; Ctsl1Cathepsin L1 ELISA kit; EC 3.4.22.15 ELISA kit; Cathepsin L ELISA kit; Major excreted protein ELISA kit; MEP ELISA kit; p39 cysteine proteinase) [Cleaved into: Cathepsin L1 heavy chain; Cathepsin L1 light chain] ELISA kit
缩写：CTSL1
Uniprot No.：P06797
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTSL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 106 | ||||
| Range % | 102-113 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of mouse CTSL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.1622.3202.2412.05825001.5731.5341.5541.37112500.9480.8920.9200.7376250.5490.5130.5310.3483120.3590.3710.3650.1821560.2540.2370.2460.063780.2240.2120.2180.03500.1800.1850.183 | ||||||
货期：3-5 working days
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