首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠组织蛋白酶K(cath-K)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTSK含量。CTSK（组织蛋白酶K）是一种在骨重塑过程中起关键作用的酶，主要在破骨细胞中表达。其研究机制涉及降解骨细胞外基质，促进骨吸收。CTSK在多种癌症中表达，可能促进肿瘤细胞增殖、侵袭和迁移。研究CTSK有助于开发新的治疗策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于大鼠骨代谢研究、骨骼疾病动物模型评估及骨靶向药物作用机制探索等科研领域，可为骨生物学、病理机制研究及药物开发提供稳定可靠的定量分析支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ctsk ELISA Kit; Cathepsin K ELISA Kit; EC 3.4.22.38 ELISA Kit
缩写：CTSK
Uniprot No.：O35186
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat cath-K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 100-107 | ||||||
| 1:2 | Average % | 99 | |||||
| Range % | 95-103 | ||||||
| 1:4 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
回收率：
| The recovery of rat cath-K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 83-89 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.8012.7652.7832.682 5002.3422.2042.2732.172 2501.3431.4061.3751.274 1250.6790.7010.6900.589 62.50.3990.4050.4020.301 31.20.2510.2420.2470.146 15.60.1660.1640.1650.064 00.1020.1000.101 | |||||||
货期：3-5 working days
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