| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human AKT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AKT2抗体的3篇参考文献示例(内容基于公开文献概括，非真实引用)：
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1. **文献名称**：*AKT2-specific antibody development and its role in pancreatic cancer progression*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种高特异性兔源AKT2单克隆抗体，验证了其在Western blot和免疫组化中的适用性，并发现AKT2在胰腺癌中高表达，与患者生存率负相关。
2. **文献名称**：*Validation of a phospho-specific AKT2 antibody for signaling pathway analysis*
**作者**：Chen L, et al.
**摘要**：文章报道了一种针对AKT2磷酸化位点(Ser474)的抗体验证，证实其在胰岛素信号通路研究中的特异性，并应用于小鼠肝脏组织的蛋白活性检测。
3. **文献名称**：*AKT2 isoform dominance in cardiac hypertrophy revealed by antibody-mediated isoform distinction*
**作者**：Garcia R, et al.
**摘要**：通过比较多种AKT2抗体的特异性，研究发现AKT2在心肌肥厚中显著上调，并揭示了抗体选择对区分AKT1/AKT2/AKT3同源蛋白的关键作用。
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**备注**：以上文献为示例，实际引用时需通过PubMed、Google Scholar等平台检索真实发表的论文，并核对作者、标题及摘要内容。
**Background of AKT2 Antibody**
The AKT2 antibody is a crucial tool for studying the protein kinase AKT2 (also known as protein kinase B beta), a member of the AKT serine/threonine kinase family. AKT2 plays a central role in the PI3K/AKT/mTOR signaling pathway, which regulates cell survival, proliferation, metabolism, and apoptosis. Among the three AKT isoforms (AKT1. AKT2. AKT3), AKT2 is particularly associated with insulin signaling and glucose homeostasis, with strong implications in metabolic disorders like diabetes and cancer. Dysregulation of AKT2 is linked to tumor progression, chemotherapy resistance, and aberrant cell growth in cancers such as ovarian, pancreatic, and breast carcinomas.
AKT2 antibodies are designed to detect and quantify AKT2 expression, phosphorylation status (e.g., at Ser474 or Thr309), or localization in tissues and cell lines. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Phospho-specific AKT2 antibodies help assess pathway activation, aiding research into targeted therapies or resistance mechanisms.
Due to high sequence homology among AKT isoforms, antibody specificity is critical. Validation via knockout cell lines or siRNA-mediated knockdown ensures minimal cross-reactivity. Clinically, AKT2 antibodies contribute to biomarker studies, exploring correlations between AKT2 overexpression and disease prognosis. Their role in elucidating AKT2's dual function in oncology and metabolism underscores their importance in both basic research and therapeutic development.
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