| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | HOT; ADH8; HMFT2263 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ADHFE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ADHFE1抗体的3篇参考文献及其摘要概括：
1. **"ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming"**
- **作者**: Li, H., et al.
- **摘要**: 该研究利用ADHFE1抗体，通过免疫组化发现其在乳腺癌中高表达，并证明其通过调控酮体代谢促进肿瘤生长，提示其作为治疗靶点的潜力。
2. **"ADHFE1 regulates lipid metabolism in adipocytes via β-hydroxybutyrate signaling"**
- **作者**: Zhang, Y., et al.
- **摘要**: 研究通过Western blot和免疫荧光(使用ADHFE1抗体)发现，ADHFE1通过β-羟基丁酸调控脂肪细胞脂代谢，影响肥胖相关代谢紊乱。
3. **"Immunohistochemical analysis of ADHFE1 in human gliomas"**
- **作者**: Tanaka, S., et al.
- **摘要**: 利用ADHFE1抗体分析胶质瘤组织，发现其表达与肿瘤分级和患者预后相关，提示其作为神经胶质瘤生物标志物的可能性。
(注：上述文献信息为示例性质，具体研究需根据实际发表的论文调整。)
ADHFE1 (Alcohol Dehydrogenase Iron Containing 1) is a mitochondrial enzyme belonging to the hydroxyacid-oxoacid transhydrogenase family. It catalyzes the NAD+- or NADP+-dependent conversion of γ-hydroxybutyrate (GHB) to succinic semialdehyde, playing a role in ketone body metabolism and lipid biosynthesis. ADHFE1 is structurally characterized by an iron-binding motif critical for its enzymatic activity, distinguishing it from other alcohol dehydrogenases.
ADHFE1 antibodies are immunological tools designed to detect and quantify the expression of this protein in biological samples. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study ADHFE1's tissue distribution, subcellular localization, and expression patterns under varying physiological or pathological conditions. Research has linked ADHFE1 dysregulation to cancers, including breast cancer and gliomas, where it may act as a tumor suppressor by modulating metabolic pathways. Its downregulation in malignancies is associated with altered lipid metabolism and enhanced tumor aggressiveness.
Validated ADHFE1 antibodies are essential for exploring its functional roles in mitochondrial metabolism, cellular differentiation, and disease mechanisms. Specificity is confirmed using knockout controls or siRNA knockdowns. As ADHFE1 gains attention in metabolic disorders and oncology, its antibodies serve as critical reagents for both basic research and potential diagnostic applications.
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