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Mouse interferon-inducible protein 10,IP-10 ELISA Kit

  • 中文名： 小鼠干扰素诱导蛋白10(IP-10/CXCL10)ELISA Kit
货号: IPD-E08183m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠干扰素诱导蛋白10(IP-10/CXCL10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL10含量。CXCL10是一个重要靶点。它在免疫调节等生理过程中发挥关键作用，参与多种疾病的发生发展。其研究机制主要围绕与受体相互作用，激活下游信号通路，影响细胞迁移、增殖等，为开发针对该靶点的疾病治疗药物提供了重要方向。试剂盒检测范围为0.312 ng/ml- 20 ng/ml，灵敏度为0.078 ng/ml。常用于研究感染性疾病、自身免疫疾病及肿瘤微环境等科研领域；适用于小鼠模型中炎症机制探索、免疫应答动态分析或药物干预效果评价等实验；适用于细胞因子网络研究、疾病模型机制解析或生物标志物筛选等科研用途本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cxcl10 ELISA kit; Crg2 ELISA kit; Ifi10 ELISA kit; Inp10 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; C7 ELISA kit; Interferon-gamma induced protein CRG-2 ELISA kit; Small-inducible cytokine B10 ELISA kit
  • 缩写：
    CXCL10
  • Uniprot No.：
    P17515
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/ml - 20 ng/ml
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP-10/CXCL10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    88-99


    1:2

    Average %

    97


    Range %

    91-102


    1:4

    Average %

    99


    Range %

    92-108


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of mouse IP-10/CXCL10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    93

    90-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    2.504

    2.634

    2.569

    2.407


    10

    1.725

    1.667

    1.696

    1.534


    5

    1.218

    1.158

    1.188

    1.026


    2.5

    0.789

    0.769

    0.779

    0.617


    1.25

    0.491

    0.501

    0.496

    0.334


    0.625

    0.367

    0.353

    0.360

    0.199


    0.312

    0.253

    0.244

    0.248

    0.087


    0

    0.158

    0.165

    0.162




  • 货期：
    3-5 working days

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