首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠CXC趋化因子配体1(CXCL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL1含量。CXCL1是重要的趋化因子，参与免疫细胞募集、炎症反应调节等过程。其表达异常与肿瘤生长、转移及多种炎症疾病发生发展相关。研究聚焦其信号传导通路，探索其在疾病进程中的作用机制，为开发靶向治疗药物提供理论基础。试剂盒检测范围为7.8 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。炎症性疾病模型构建、免疫调控机制探索、药物疗效评价、研究CXCL1在感染、自身免疫疾病或肿瘤微环境中的作用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cxcl1 ELISA Kit; Gro ELISA Kit; Gro1 ELISA Kit; Mgsa ELISA Kit; Scyb1Growth-regulated alpha protein ELISA Kit; C-X-C motif chemokine 1 ELISA Kit; Platelet-derived growth factor-inducible protein KC ELISA Kit; Secretory protein N51) [Cleaved into: KC(5-72 ELISA Kit; Hematopoietic synergistic factor ELISA Kit; HSF ELISA Kit; KC-T)] ELISA Kit
缩写：CXCL1
Uniprot No.：P12850
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 81-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 85-103 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-103 | |||||
回收率：
| The recovery of mouse CXCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-101 | ||||
| EDTA plasma (n=4) | 94 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 5002.8132.7642.7892.641 2502.0101.9881.9991.851 1251.2541.2911.2731.125 62.50.7470.7820.7650.617 31.20.5910.5820.5870.439 15.60.3260.3170.3220.174 7.80.2150.2280.2220.074 00.1450.1510.148 | ||||||
货期：3-5 working days
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