| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | VPS34; hVps34 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PIK3C3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PIK3C3抗体的3-4条参考文献示例，包含文献名称、作者及摘要内容的简要概括：
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1. **文献名称**：*"The Role of Vps34 Kinase in Autophagosome Formation"*
**作者**：Noboru Mizushima, Tamotsu Yoshimori
**摘要内容**：该研究通过使用特异性PIK3C3抗体(VPS34抗体)，结合免疫荧光和免疫印迹技术，揭示了VPS34在自噬体形成中的关键作用。实验表明，抑制PIK3C3活性会显著阻断LC3脂化及自噬体成熟，证实了其在自噬信号通路中的核心地位。
2. **文献名称**：*"Characterization of PIK3C3-Specific Antibodies for Autophagy Studies"*
**作者**：Sharon A. Tooze, David C. Rubinsztein
**摘要内容**：本文系统评估了多种商业化PIK3C3抗体的特异性及适用性，通过敲除细胞系验证抗体可靠性，并展示了其在免疫沉淀和共聚焦显微镜中的应用，为自噬研究中的抗体选择提供了参考。
3. **文献名称**：*"PIK3C3/VPS34 Dysregulation Promotes Tumorigenesis in Breast Cancer"*
**作者**：Beth Levine, Hong-Gang Wang
**摘要内容**：研究利用PIK3C3抗体检测乳腺癌组织中VPS34蛋白的表达水平，发现其表达异常与自噬失调及肿瘤进展相关，提示PIK3C3可能成为癌症治疗的潜在靶点。
4. **文献名称**：*"Functional Genomic Analysis of the Beclin 1-VPS34 Complex in Nutrient Stress"*
**作者**：Jun-Lin Guan, Qing Zhong
**摘要内容**：通过免疫共沉淀结合PIK3C3抗体，研究揭示了饥饿条件下Beclin 1-VPS34复合物的动态调控机制，证实该复合物在细胞应对代谢压力时调控自噬活性的重要性。
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以上文献为示例，实际引用时请核对具体论文内容及发表信息，建议通过PubMed或Google Scholar以关键词“PIK3C3 antibody”或“VPS34 antibody”获取最新研究。
**Background of PIK3C3 Antibody**
PIK3C3 (Phosphatidylinositol 3-Kinase Catalytic Subunit Type 3), also known as Vps34 (vacuolar protein sorting 34), is a lipid kinase that plays a central role in intracellular membrane trafficking, autophagy, and cellular signaling. It belongs to the class III PI3K family and functions by generating phosphatidylinositol 3-phosphate (PI3P), a lipid second messenger essential for recruiting effector proteins to specific membrane compartments. PIK3C3 forms distinct complexes with regulatory subunits such as Vps15. Beclin-1. and ATG14L or UVRAG, which dictate its involvement in autophagy initiation, endosomal sorting, or other vesicular trafficking pathways.
Antibodies targeting PIK3C3 are critical tools for studying its expression, localization, and functional roles in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate PIK3C3-protein interactions, kinase activity, and regulatory mechanisms. Dysregulation of PIK3C3 is implicated in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, making its detection and analysis vital for understanding disease pathogenesis. Researchers also utilize PIK3C3 antibodies to explore therapeutic strategies, including autophagy modulation in chemotherapy or targeting PI3P signaling pathways. Specificity and validation of these antibodies are crucial, given the structural similarities among PI3K family members and their diverse cellular functions.
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