首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠CX3C趋化因子受体1(CX3CR1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CX3CR1含量。CX3CR1，全称chemokine (C-X3-C motif) receptor 1，是一种趋化因子受体，其与CX3CL1结合后介导免疫细胞趋化或粘附，与动脉粥样硬化、神经炎症、神经退行性疾病、癌症等密切相关。研究显示，CX3CR1通过调节巨噬细胞极化影响肾间质纤维化，其信号识别与转导机制正被深入研究。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于基础科研领域中对CX3CR1表达调控机制的研究，例如在神经系统疾病模型（如阿尔茨海默病、帕金森病）、免疫微环境分析或药物干预实验中的分子水平检测，为探索疾病机制或潜在治疗靶点提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cx3cr1; Rbs11; CX3C chemokine receptor 1; C-X3-C CKR-1; CX3CR1; Fractalkine receptor
缩写：CX3CR1
Uniprot No.：P35411
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CX3CR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 99-108 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-98 | |||||
回收率：
| The recovery of rat CX3CR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-94 | ||||
| EDTA plasma (n=4) | 107 | 99-111 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.1912.1242.1582.0357501.4451.4521.4491.3263751.0881.0511.0700.947187.50.6340.6220.6280.505940.3720.3890.3810.258470.2880.2780.2830.16023.50.2100.2070.2090.08600.1240.1220.123 | ||||||
货期：3-5 working days
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