| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | EIF2C4 |
| WB Predicted band size | 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human AGO4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于AGO4抗体的参考文献摘要示例(文献信息为模拟虚构，仅供格式参考)：
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1. **文献名称**: *AGO4 mediates DNA methylation through siRNA-guided chromatin silencing in Arabidopsis*
**作者**: Zilberman, D., et al.
**摘要**: 本研究利用AGO4特异性抗体通过免疫共沉淀(IP)和ChIP-seq技术，揭示了拟南芥中AGO4蛋白在RNA导向的DNA甲基化中的作用机制，证明其与siRNA协同调控转座子区域的表观遗传沉默。
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2. **文献名称**: *A mammalian AGO4 complex regulates heterochromatin formation*
**作者**: Qi, Y., et al.
**摘要**: 通过Western blot和免疫荧光实验，作者使用兔源AGO4多克隆抗体验证了哺乳动物细胞中AGO4蛋白在异染色质形成中的功能，发现其与HP1蛋白互作并维持基因组稳定性。
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3. **文献名称**: *AGO4 antibody-based profiling reveals dynamic expression in hepatocellular carcinoma*
**作者**: Zheng, L., et al.
**摘要**: 该研究开发了一种高特异性小鼠单克隆AGO4抗体，通过免疫组化分析肝癌样本，发现AGO4的异常表达与肿瘤侵袭性相关，提示其作为潜在生物标志物的价值。
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(注：以上文献为示例性内容，实际引用需根据具体研究核实。)
The AGO4 (Argonaute 4) antibody is a crucial tool for studying RNA-mediated gene silencing and epigenetic regulation. AGO4 belongs to the Argonaute protein family, which plays a central role in RNA interference (RNAi) pathways by binding small non-coding RNAs (e.g., siRNAs or miRNAs) to guide sequence-specific silencing of target genes. Specifically, AGO4 is a key component of the RNA-directed DNA methylation (RdDM) pathway in plants, where it interacts with 24-nt small interfering RNAs (siRNAs) to establish de novo DNA methylation and maintain heterochromatin, thereby regulating gene expression and transposon repression. In mammals, AGO4 is less characterized but has been implicated in nuclear RNAi processes, chromatin remodeling, and potential roles in cancer or stem cell biology.
The AGO4 antibody is widely used to detect endogenous AGO4 protein levels, localization, and interactions in various experimental models, including *Arabidopsis*, human, and mouse. It enables techniques like Western blotting, immunoprecipitation, and immunofluorescence, aiding research into epigenetic mechanisms, gene silencing, and developmental regulation. Commercial AGO4 antibodies are typically raised against conserved epitopes, such as the N-terminal or PIWI domains, and validated for specificity against other Argonaute family members (e.g., AGO1-3). Its applications span plant epigenetics, mammalian gene regulation studies, and explorations of RNAi-based therapeutic strategies.
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