| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | BIG2; PVNH2; dJ1164I10.1 |
| WB Predicted band size | 202 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARFGEF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **ARFGEF2抗体** 的模拟参考文献示例(可能为虚构，建议通过学术数据库核实)：
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1. **文献名称**：*ARFGEF2 regulates neurodevelopmental disorders through vesicular trafficking*
**作者**：Sheen, V. et al.
**摘要**：本研究利用ARFGEF2特异性抗体，通过免疫组化和Western blot分析，揭示了ARFGEF2基因突变导致神经元迁移缺陷的分子机制。抗体验证显示ARFGEF2在皮层神经元中高表达，其功能缺失与脑畸形相关。
2. **文献名称**：*ARFGEF2 controls Golgi-ER retrograde transport by activating ARF1*
**作者**：Boman, A.L. et al.
**摘要**：通过ARFGEF2抗体的免疫沉淀实验，作者发现其作为鸟嘌呤核苷酸交换因子(GEF)激活ARF1.调控高尔基体-内质网膜运输。抗体阻断实验证实ARFGEF2对细胞器形态维持至关重要。
3. **文献名称**：*ARFGEF2 expression correlates with cancer cell invasiveness*
**作者**：Xu, J. et al.
**摘要**：在多种癌细胞系中，ARFGEF2抗体检测显示其过表达与侵袭性表型相关。敲低ARFGEF2后，通过抗体标记观察到细胞迁移能力显著下降，提示其作为癌症治疗靶点的潜力。
4. **文献名称**：*Functional analysis of ARFGEF2 in synaptic vesicle recycling*
**作者**：Shiba, Y. et al.
**摘要**：利用ARFGEF2特异性抗体进行免疫荧光定位，发现其富集于突触前膜。功能研究表明，ARFGEF2通过调节囊泡循环影响神经递质释放，抗体介导的抑制实验进一步验证了这一机制。
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**注意**：以上文献为示例性质，实际研究需通过 **PubMed** 或 **Google Scholar** 检索关键词(如“ARFGEF2 antibody”、“BIG2 protein”)获取真实文献。
The ARFGEF2 antibody is a crucial tool for studying the ADP-ribosylation factor guanine nucleotide-exchange factor 2 (ARFGEF2), a protein involved in intracellular vesicular trafficking and membrane remodeling. ARFGEF2. also known as BIG2. facilitates the activation of ADP-ribosylation factors (ARFs) by promoting the exchange of GDP for GTP, a process essential for recruiting coat proteins during vesicle formation. This protein plays a vital role in neuronal development, Golgi apparatus maintenance, and neurotransmitter release. Dysregulation of ARFGEF2 has been linked to neurodevelopmental disorders, including periventricular heterotopia, where mutations disrupt neuronal migration.
The ARFGEF2 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect the protein’s expression, localization, and interactions in various tissues, particularly the brain. Researchers employ it to investigate ARFGEF2’s role in cellular processes, such as autophagy, receptor trafficking, and synaptic function, as well as its implications in diseases like cancer and neurological conditions. Specificity and validation (e.g., knockout controls) are critical to ensure accurate detection, given potential cross-reactivity with homologous proteins like BIG1 (ARFGEF1). Commercial antibodies are typically raised in rabbits or mice, targeting epitopes within conserved regions of human ARFGEF2. with applications spanning basic research and clinical diagnostics.
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