| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | CENTD2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ARAP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇涉及ARAP1抗体的代表性文献示例(注：文献为虚构，仅作格式参考，实际文献需通过学术数据库检索获取)：
1. **文献名称**: *"ARAP1 modulates insulin signaling through interaction with phosphatidylinositol 3-kinase"*
**作者**: Smith J, et al.
**摘要**: 本研究利用ARAP1特异性抗体进行免疫沉淀和Western blot分析，发现ARAP1通过与PI3K相互作用调控胰岛素受体下游信号传导，影响葡萄糖代谢。
2. **文献名称**: *"ARAP1 expression in prostate cancer correlates with tumor progression"*
**作者**: Lee H, et al.
**摘要**: 通过ARAP1抗体进行免疫组化染色，发现ARAP1在前列腺癌组织中高表达，且与肿瘤侵袭性和患者预后不良相关，提示其作为潜在生物标志物的价值。
3. **文献名称**: *"ARAP1 regulates endosomal trafficking via its GTPase-activating protein domain"*
**作者**: Chen R, et al.
**摘要**: 利用ARAP1抗体进行亚细胞定位研究，证实ARAP1通过其GAP结构域调控Arf6和RhoA活性，影响内体膜运输和细胞骨架重组。
如需真实文献，建议在PubMed或Google Scholar检索关键词“ARAP1 antibody”+“function”/“disease”等，并筛选应用该抗体的实验研究。
The ARAP1 (ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1) antibody is a crucial tool for studying the function and expression of the ARAP1 protein, a multidomain signaling molecule involved in regulating cellular processes such as cytoskeletal reorganization, membrane trafficking, and cell adhesion. ARAP1 acts as a dual GTPase-activating protein (GAP) for both Arf and Rho family GTPases, modulating their activity to control intracellular signaling pathways. It plays roles in insulin signaling, endocytic trafficking, and cell migration, with implications in metabolic disorders, cancer, and immune regulation.
ARAP1 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect ARAP1 expression levels and localization in tissues or cultured cells. Researchers employ these antibodies to investigate ARAP1's involvement in diseases such as diabetes, where ARAP1 polymorphisms are linked to insulin resistance, or in cancer metastasis due to its regulatory effects on cell motility. Commercial ARAP1 antibodies are typically raised against specific epitopes, often within its N-terminal region or functional domains, and validated for species reactivity (e.g., human, mouse). Proper validation via knockout controls or siRNA knockdown is essential to ensure specificity, given ARAP1's structural complexity and homology with other GAP proteins.
×
