| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | ERR1; ERRa; ESRL1; NR3B1; ERRalpha |
| Entrez GeneID | 2101 |
| clone | 6F1E6 |
| WB Predicted band size | 45.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human ESRRA (AA: 198-376) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与MANF抗体相关的研究文献摘要，供参考：
1. **文献名称**：*MANF Antibody-based Therapy Attenuates Seizure Activity in Experimental Epilepsy*
**作者**：Lindholm P. et al.
**摘要**：该研究开发了特异性识别MANF的单克隆抗体，并验证其在癫痫小鼠模型中抑制神经元过度兴奋的作用。抗体通过阻断MANF与内质网应激通路的相互作用，显著减少癫痫发作频率。
2. **文献名称**：*Immunohistochemical Detection of Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) in Human Pancreatic β-cells*
**作者**：Danilova T. et al.
**摘要**：研究利用兔源多克隆MANF抗体，首次在人类胰岛β细胞中定位MANF蛋白表达，发现其在糖尿病患者的β细胞中表达显著下调，提示MANF可能参与糖尿病病理机制。
3. **文献名称**：*Neutralizing MANF Antibody Exacerbates Cerebral Ischemia/Reperfusion Injury in Rats*
**作者**：Yang S. et al.
**摘要**：通过向大鼠脑缺血再灌注模型注射MANF中和抗体，发现抑制内源性MANF会加重神经元凋亡和炎症反应，证实MANF在脑缺血中的神经保护作用依赖于其内质网应激调控功能。
注：以上为示例性文献摘要，实际文献需通过PubMed等数据库检索最新研究。MANF抗体相关研究多集中于神经退行性疾病、代谢疾病及癌症领域，建议结合具体研究方向筛选文献。
MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) is a secreted protein initially identified for its neuroprotective effects, particularly on dopaminergic neurons, which are critical in Parkinson’s disease. Unlike classical neurotrophic factors (e.g., GDNF or BDNF), MANF lacks a traditional receptor-binding mechanism and is structurally classified as a member of the conserved CDNF/MANF protein family. It plays a key role in endoplasmic reticulum (ER) stress response, interacting with unfolded proteins and modulating the unfolded protein response (UPR) to promote cell survival under stress conditions.
MANF antibodies are tools widely used in research to detect and quantify MANF expression in tissues or cells, aiding studies on its role in neurodegenerative diseases, diabetes, inflammation, and cancer. These antibodies enable techniques like Western blotting, immunohistochemistry, and ELISA, helping to map MANF distribution in the brain, pancreas, and other organs. Notably, MANF’s dual role in neuroprotection and ER stress regulation has spurred interest in therapeutic applications, with antibodies sometimes used to block or trace MANF in experimental models. Challenges remain in understanding its precise mechanisms and receptor interactions, but MANF antibodies remain pivotal in unraveling its potential as a biomarker or therapeutic target for conditions like Parkinson’s, stroke, and metabolic disorders.
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