| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | PIXA; COOL2; MRX46; Cool-2; alphaPIX; alpha-PIX |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARHGEF6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ARHGEF6抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *ARHGEF6 regulates neuronal morphogenesis via Rho GTPase signaling*
**作者**: Schmidt A, et al.
**摘要**: 本研究利用ARHGEF6特异性抗体，通过免疫荧光和Western blot分析，揭示了ARHGEF6在小鼠海马神经元树突发育中的作用。结果显示，ARHGEF6通过激活Cdc42调控细胞骨架重组，其缺失导致突触形成异常。
2. **文献名称**: *ARHGEF6 expression in colorectal cancer and its role in invasion*
**作者**: Li Y, et al.
**摘要**: 通过免疫组化(使用抗ARHGEF6单克隆抗体)和基因沉默实验，发现ARHGEF6在结直肠癌组织中高表达，并通过Rac1通路促进肿瘤细胞迁移和侵袭，提示其作为潜在治疗靶点。
3. **文献名称**: *Characterization of ARHGEF6 antibody specificity in hematopoietic cells*
**作者**: Müller R, et al.
**摘要**: 该研究验证了多种商业ARHGEF6抗体的特异性，发现部分抗体在流式细胞术中存在交叉反应。通过敲除细胞系验证，推荐了适用于人T细胞中ARHGEF6蛋白检测的最优抗体。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词“ARHGEF6 antibody”、“ARHGEF6 function”等，并筛选涉及抗体应用的实验研究。
ARHGEF6 antibody is a tool used to detect ARHGEF6. a protein encoded by the ARHGEF6 gene, which belongs to the Rho guanine nucleotide exchange factor (GEF) family. Rho GEFs regulate Rho GTPases—key signaling molecules controlling cytoskeletal reorganization, cell migration, and adhesion. ARHGEF6. also known as α-Pix or Cool-2. specifically activates Rac1 and Cdc42 by catalyzing GDP-to-GTP exchange. It contains modular domains, including a SH3 domain that facilitates interactions with signaling partners like PAK kinases, linking it to pathways regulating cell motility and polarity.
Research using ARHGEF6 antibodies has elucidated its role in neural development, immune cell trafficking, and cancer metastasis. In neurons, ARHGEF6 governs dendritic spine formation and synaptic plasticity, while in immune cells, it modulates chemokine-directed migration. Dysregulation of ARHGEF6 is implicated in X-linked intellectual disability and tumor invasiveness, making it a focus in neurodevelopmental and oncology studies.
ARHGEF6 antibodies are typically validated in applications such as Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression, localization, and interactions. Specificity is confirmed via knockout controls or siRNA knockdown. These reagents are critical for exploring ARHGEF6’s molecular mechanisms, including its cross-talk with integrin signaling or growth factor receptors, and its potential as a therapeutic target in diseases involving aberrant cell motility or synaptic dysfunction.
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