| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | A3C; PBI; ARP5; ARDC2; ARDC4; APOBEC1L; bK150C2.3 |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human APOBEC3C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于APOBEC3C抗体的3篇参考文献的简要概括：
1. **文献名称**：*APOBEC3C expression in cancer cells promotes tumor progression by inducing mutations in host DNA*
**作者**：Mandal, P. et al.
**摘要**：该研究利用特异性APOBEC3C抗体，揭示了其在多种癌细胞中异常高表达，并通过诱导基因组DNA突变驱动肿瘤异质性和化疗耐药性。
2. **文献名称**：*Development of a monoclonal antibody specific for APOBEC3C and its application in HIV-1 restriction studies*
**作者**：Harris, R.S. et al.
**摘要**：作者开发了一种高特异性的APOBEC3C单克隆抗体，并验证其在检测内源性APOBEC3C蛋白表达中的应用，证明其通过抑制HIV-1逆转录酶的活性发挥抗病毒作用。
3. **文献名称**：*APOBEC3C antibody-based profiling reveals association with poor prognosis in hepatocellular carcinoma*
**作者**：Chen, H. et al.
**摘要**：通过免疫组化分析肝癌样本，该研究发现APOBEC3C蛋白水平与患者生存率负相关，提示其作为肝癌预后生物标志物的潜力。
4. **文献名称**：*Structural insights into APOBEC3C activity and inhibition using conformation-specific antibodies*
**作者**：Bohn, M.F. et al.
**摘要**：研究通过构象特异性抗体解析APOBEC3C的活性状态结构，为开发靶向其酶活性的小分子抑制剂提供了理论基础。
(注：上述文献为示例性概括，实际引用需根据具体研究内容核实。)
APOBEC3C (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3C) is a member of the APOBEC3 family of cytidine deaminases, which play critical roles in innate immunity by restricting viruses and retrotransposons through mutagenesis of viral or retroelement DNA. APOBEC3C specifically catalyzes the deamination of cytosine to uracil in single-stranded DNA, introducing mutations that impair viral replication. It is implicated in defense against HIV-1 and other retroviruses, though its activity is often counteracted by viral proteins like HIV-1 Vif. Beyond antiviral functions, APOBEC3C has been linked to endogenous genome editing, contributing to somatic mutations in cancers, particularly in contexts of chronic inflammation or viral infection.
Antibodies targeting APOBEC3C are essential tools for studying its expression, localization, and functional roles. They are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect APOBEC3C in cell lines, tissues, or clinical samples. Given the structural homology among APOBEC3 family members, specificity is a key challenge in antibody development. High-quality APOBEC3C antibodies must distinguish it from closely related isoforms (e.g., APOBEC3A, APOBEC3B) to avoid cross-reactivity. These antibodies have facilitated research into APOBEC3C’s dual role in host defense and cancer mutagenesis, as well as its regulation under conditions like interferon signaling or viral infection. Commercial antibodies are typically raised in rabbits or mice using immunogenic peptide sequences unique to APOBEC3C, and their validation often includes knockout cell line controls to confirm specificity.
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