| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ADTB1; BAM22; AP105A; CLAPB2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human AP1B1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AP1B1抗体的3篇参考文献示例(内容基于已有研究概括，非真实文献，仅供参考)：
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1. **文献名称**：*AP1B1 regulates basolateral protein sorting in polarized epithelial cells*
**作者**：Folsch H, et al.
**摘要**：该研究利用AP1B1特异性抗体，通过免疫荧光和免疫印迹技术，揭示了AP1B1在极化上皮细胞中对基底外侧膜蛋白分选的关键作用，并证明其缺失会导致蛋白运输异常。
2. **文献名称**：*The adaptor protein AP1B1 interacts with clathrin to mediate endosomal trafficking*
**作者**：Nakatsu F, et al.
**摘要**：通过AP1B1抗体共沉淀实验，作者阐明了AP1B1与网格蛋白(clathrin)的相互作用机制，及其在内体运输和细胞膜循环过程中的功能。
3. **文献名称**：*AP1B1 deficiency impairs cell polarity and promotes cancer metastasis*
**作者**：Marquez C, et al.
**摘要**：研究使用AP1B1抗体检测肿瘤组织中AP1B1的表达水平，发现其低表达与上皮-间充质转化(EMT)和癌症转移显著相关，提示AP1B1可能作为抑癌分子发挥作用。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“AP1B1 antibody”或“AP1B1 function”，并筛选近年高引文章。
The AP1B1 antibody targets the beta-1 subunit of the Adaptor Protein complex 1 (AP-1), a heterotetramer involved in clathrin-mediated vesicular trafficking. AP-1 complexes facilitate protein sorting between the trans-Golgi network (TGN) and endosomes, playing a critical role in maintaining cellular polarity, particularly in epithelial cells. AP1B1. a tissue-specific isoform of the AP-1 beta subunit, is predominantly expressed in polarized epithelial cells and certain cancer cell lines, where it directs basolateral sorting of membrane proteins. This function is essential for establishing asymmetric protein distribution, crucial for organ development and barrier formation.
AP1B1 antibodies are widely used in research to study intracellular trafficking mechanisms, epithelial cell biology, and diseases linked to polarity defects, such as cancer metastasis or genetic disorders like microvillus inclusion disease. These antibodies enable detection of AP1B1 expression levels, localization via immunofluorescence, or validation of protein interactions in co-immunoprecipitation assays. Commercial AP1B1 antibodies are typically raised against specific epitopes, such as the C-terminal region, and validated for applications including Western blotting and immunohistochemistry. Dysregulation of AP1B1 has been implicated in disrupted cell polarity and aberrant protein trafficking, making it a biomarker of interest in epithelial-derived tumors and metabolic disorders. Research using AP1B1 antibodies continues to uncover its regulatory roles in membrane dynamics and disease pathophysiology.
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