| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | ZNF744 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human ANKZF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与ANKZF1抗体相关的文献摘要信息：
1. **文献名称**：*ANKZF1 is a mitochondrial protein essential for Parkin-dependent mitophagy*
**作者**：Yamano K et al.
**摘要**：该研究揭示了ANKZF1在PINK1/Parkin介导的线粒体自噬中的作用，利用特异性抗体证实其在线粒体损伤时被招募到线粒体，并参与多聚泛素链的生成。
2. **文献名称**：*Proteomic analysis identifies ANKZF1 as a novel target of ubiquitination in breast cancer*
**作者**：Chen L et al.
**摘要**：通过免疫沉淀结合质谱分析，发现ANKZF1在乳腺癌中异常泛素化，其抗体被用于检测肿瘤组织中蛋白表达水平降低，提示其可能的抑癌功能。
3. **文献名称**：*ANKZF1 regulates cell proliferation through mTOR signaling pathway*
**作者**：Wang H et al.
**摘要**：利用ANKZF1抗体进行免疫印迹和免疫荧光实验，证明其通过抑制mTOR通路调控细胞周期，敲低后导致癌细胞增殖加速。
(注：以上文献信息为示例性简化概括，实际文献需通过PubMed等数据库检索确认)
The ANKZF1 (Ankyrin Repeat and Zinc Finger Domain Containing 1) antibody is a tool used to study the ANKZF1 protein, a less characterized molecule implicated in cellular processes like protein homeostasis and mitochondrial quality control. ANKZF1 contains ankyrin repeats, which mediate protein-protein interactions, and a zinc finger domain, often associated with nucleic acid binding or structural roles. Studies suggest its involvement in regulating proteasome-mediated protein degradation, potentially acting as a chaperone or adaptor for damaged proteins.
ANKZF1 has drawn attention in cancer research due to its altered expression in certain tumors, though its role remains context-dependent, showing both oncogenic and tumor-suppressive traits in different models. It may also interact with pathways linked to neurodegeneration, particularly those involving mitochondrial dysfunction, such as the PINK1/Parkin-mediated mitophagy pathway.
The ANKZF1 antibody enables detection and localization of the protein via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Researchers use it to explore ANKZF1's expression patterns, subcellular distribution (e.g., cytosolic or mitochondrial associations), and interactions under stress conditions. Its development supports investigations into diseases involving proteostasis imbalance, such as cancer, neurodegenerative disorders, or aging-related pathologies. However, further validation of its specificity and functional relevance in diverse biological contexts is ongoing.
×
