首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Ⅱ型胶原C端肽(CTX-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTX-Ⅱ含量。试剂盒检测范围为3.12 pg/mL-200 pg/mL，在骨关节炎模型研究、软骨退行性病变机制探索及药物干预效果评估中具有重要应用价值；支持科研人员高效完成软骨代谢相关疾病的动物模型构建、病理机制分析以及潜在治疗靶点的验证工作；为关节炎、骨质疏松等骨骼系统疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C-telopeptide of type 2 collagen(CTX-2),_x000D_ C-telopeptide of type II collagen(CTX-II)
缩写：CTX-Ⅱ
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 86-95 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:80 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:160 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of mouse CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-96 | ||||
| EDTA plasma (n=4) | 94 | 91-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 2002.2902.2562.2732.112 1001.6711.6661.6691.508 501.1431.1321.1380.977 250.7180.7010.7100.549 12.50.4120.4270.4200.259 6.250.3010.2850.2930.132 3.120.2280.2210.2250.064 00.1590.1630.161 | ||||||
货期：3-5 working days
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