| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | NR1I1; PPP1R163 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human VDR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NUAK1抗体的参考文献示例(内容为模拟概括，供参考)：
1. **文献名称**：*NUAK1 promotes tumor metastasis through transcriptional activation of fibronectin*
**作者**：Banales JM 等
**摘要**：研究揭示了NUAK1通过调控纤维连接蛋白表达促进肿瘤转移的机制，实验中利用NUAK1抗体进行Western blot和免疫组化验证其蛋白表达水平。
2. **文献名称**：*AMPK-related kinase NUAK1 regulates cellular energy homeostasis via autophagy*
**作者**：Zheng L 等
**摘要**：探讨NUAK1在细胞能量代谢和自噬中的作用，通过NUAK1特异性抗体检测其在营养缺乏条件下的蛋白表达变化及亚细胞定位。
3. **文献名称**：*Development of a monoclonal antibody targeting NUAK1 for kinase activity studies*
**作者**：Suzuki T 等
**摘要**：报道了一种高特异性NUAK1单克隆抗体的开发，并验证其在免疫沉淀(IP)和免疫荧光中的适用性，用于研究NUAK1激酶活性与信号通路关联。
4. **文献名称**：*NUAK1 upregulation in Alzheimer's disease models correlates with tau pathology*
**作者**：Hou X 等
**摘要**：分析NUAK1在阿尔茨海默病模型中的表达变化，使用NUAK1抗体进行脑组织切片染色，发现其与tau蛋白磷酸化程度呈正相关。
(注：以上文献信息为示例性概括，实际引用需以真实发表文献为准。)
NUAK1 (NUAK family kinase 1), also known as ARK5 (AMPK-related protein kinase 5), is a serine/threonine kinase belonging to the AMP-activated protein kinase (AMPK) family. It plays a critical role in cellular stress responses, energy homeostasis, and cell proliferation by regulating pathways such as the LKB1-AMPK signaling axis, mTOR, and Hippo. NUAK1 is involved in diverse physiological processes, including cytoskeletal organization, apoptosis, and autophagy, and has been implicated in cancer progression, particularly in tumor metastasis and chemoresistance. Its expression is often upregulated in cancers like breast, colon, and glioblastoma, correlating with poor prognosis.
NUAK1 antibodies are essential tools for studying its expression, localization, and functional interactions in both normal and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) to investigate NUAK1's role in signaling networks. Researchers also employ them to explore NUAK1's regulatory mechanisms, including phosphorylation events (e.g., at Thr211) and its interplay with binding partners like MYPT1 or PP1β. Commercial NUAK1 antibodies are typically validated for specificity across human, mouse, and rat samples, with monoclonal and polyclonal variants available. Their applications extend to drug discovery, particularly in targeting NUAK1 for anticancer therapies, and in elucidating its contributions to metabolic disorders or neurodegenerative diseases.
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