首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅱ型胶原C端肽(CTX-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CTX-Ⅱ含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒适用于体外定量分析，可支持科研领域对软骨代谢机制、药物干预效果及疾病动物模型的评估；在骨关节退行性病变、类风湿性关节炎等软骨损伤相关疾病的研究中具有重要价值；为软骨健康相关的分子机制研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C-telopeptide of type 2 collagen,CTX-2,_x000D_ C-telopeptide of type II collagen,CTX-II
缩写：CTX-Ⅱ
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 87-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of rat CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-98 | ||||
| EDTA plasma (n=4) | 95 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.2322.2022.2172.076 101.4581.4471.4531.312 50.8880.9000.8940.753 2.50.6350.6210.6280.487 1.250.3750.3620.3690.228 0.6250.2940.2880.2910.150 0.3120.2380.2270.2330.092 00.1420.1400.141 | ||||||
货期：3-5 working days
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