首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠趋化因子(FK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CX3CL1含量。CX3CL1是一种趋化因子，在免疫系统、神经系统等方面有重要作用。其异常表达与多种疾病如炎症、肿瘤等密切相关。研究机制主要聚焦于其与受体CX3CR1相互作用，介导细胞迁移、黏附等，为相关疾病治疗提供潜在靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于体外实验研究，如炎症性疾病模型构建、免疫细胞功能分析、药物干预效果评估等场景。科研人员可通过在动物实验模型中动态监测FK表达水平，为探究炎症机制、免疫调控网络及潜在治疗靶点提供可靠工具。适用于实验室常规检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cx3cl1; Cx3c; Fkn; Scyd1; Fractalkine; FK; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1
缩写：CX3CL1
Uniprot No.：O35188
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 88-94 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-105 | |||||
回收率：
| The recovery of mouse FK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-94 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.6582.7602.7092.52640002.3142.3562.3352.15220001.7031.6821.6931.51010000.9550.9010.9280.7455000.5420.6250.5840.4012500.4130.4580.4360.2531250.2910.2720.2820.09900.1880.1780.183 | ||||||
货期：3-5 working days
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