首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠趋化因子(FK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CX3CL1含量。CX3CL1，也称为Fractalkine或Neurotactin，是一种CX3C趋化因子家族成员，具有粘蛋白样糖基化柄，可作为可溶性或膜结合趋化因子存在。其受体CX3CR1是一种7次跨膜受体，介导CX3CL1的粘附和迁移功能，参与动脉粥样硬化、肾小球肾炎等多种临床疾病的发病机制。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可借助本产品探究FK在炎症性疾病动物模型中的动态表达、评估药物干预对趋化因子通路的调控作用，或用于免疫微环境相关机制研究，为心血管疾病、创伤修复等领域的科研实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cx3cl1; Acc1; Fkn; Scyd1; Fractalkine; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1
缩写：CX3CL1
Uniprot No.：O55145
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 87-95 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 82-88 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 84-94 | ||||||
回收率：
| The recovery of rat FK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 97-106 | |||||
| EDTA plasma (n=4) | 92 | 89-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected201.9091.9981.9541.784101.1921.1851.1891.01950.7340.7640.7490.5792.50.5440.5220.5330.3631.250.3410.3640.3530.1830.6250.2770.2890.2830.1130.3120.2110.2240.2180.04800.1650.1740.170 | |||||||
货期：3-5 working days
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