| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | GTRAP48; PDZ-RHOGEF |
| WB Predicted band size | 168 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ARHGEF11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ARHGEF11抗体的3篇参考文献，按文献内容及抗体应用方向整理：
1. **文献名称**：*ARHGEF11 promotes proliferation and epithelial-mesenchymal transition in hepatocellular carcinoma through activation of β-catenin pathway*
**作者**：Li X, Zhang Y, Liu Q, et al.
**摘要**：该研究利用ARHGEF11抗体(Western blot和免疫组化)证实其在肝癌组织中高表达，并通过激活β-catenin信号通路促进肿瘤细胞增殖和转移。
2. **文献名称**：*ARHGEF11 interacts with Scribble to regulate cell polarity and migration in colorectal cancer cells*
**作者**：Wang H, Chen J, Deng Y, et al.
**摘要**：通过ARHGEF11抗体免疫共沉淀(Co-IP)实验，揭示了其与极性蛋白Scribble的相互作用，调控结直肠癌细胞极性和迁移，依赖RhoA/ROCK通路。
3. **文献名称**：*A novel role of ARHGEF11 in regulating glucose metabolism via mTORC1 signaling*
**作者**：Kim S, Park J, Lee K.
**摘要**：研究使用ARHGEF11抗体进行免疫荧光定位，发现其通过mTORC1通路影响胰岛β细胞的葡萄糖代谢功能，为糖尿病机制提供新靶点。
---
**备注**：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science以“ARHGEF11 antibody”或“ARHGEF11 function”为关键词检索最新论文。若需具体文献，可补充研究背景进一步筛选。
ARHGEF11 (Rho Guanine Nucleotide Exchange Factor 11), also known as PDZ-RhoGEF, is a member of the RhoGEF family that activates Rho GTPases by facilitating the exchange of GDP for GTP. It plays a critical role in regulating cytoskeletal reorganization, cell motility, and signaling pathways linked to G protein-coupled receptors (GPCRs). The protein contains a regulator of G-protein signaling (RGS) domain and a PDZ-binding motif, enabling interactions with various signaling molecules and scaffolding proteins. Dysregulation of ARHGEF11 has been implicated in pathological processes, including cancer metastasis, cardiovascular diseases, and neurological disorders.
ARHGEF11 antibodies are essential tools for studying its expression, localization, and function in cellular and disease models. These antibodies are commonly used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect ARHGEF11 in tissues or cultured cells. Researchers also employ them to investigate its role in RhoA-mediated signaling cascades and cross-talk with other pathways, such as Wnt or Hippo. Commercially available antibodies are typically raised against specific epitopes, often within conserved regions like the RGS or DH/PH domains. Validation includes testing for specificity via knockout cell lines or siRNA knockdown. Understanding ARHGEF11's mechanisms through antibody-based research may aid in developing therapeutic strategies targeting Rho GTPase pathways in diseases like hypertension or invasive cancers.
×
