首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠基质细胞衍生因子1β(SDF-1β/CXCL12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CXCL12含量。CXCL12即基质细胞衍生因子-1，趋化因子家族成员，在胚胎发育、组织稳态等生理过程中发挥重要作用。研究发现其主要通过与CXCR4和CXCR7结合传导信号，在肿瘤进展、炎症反应等方面机制受关注，是潜在治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，广泛应用于科研领域，包括探究炎症性疾病机制、肿瘤转移微环境中的趋化因子调控网络、干细胞定向迁移模型建立等基础研究，也可用于评估药物干预或基因编辑对CXCL12信号通路的影响，为体外实验和动物模型研究提供可靠的定量检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cxcl12 ELISA Kit; Sdf1 ELISA Kit; Stromal cell-derived factor 1 ELISA Kit; SDF-1 ELISA Kit; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 ELISA Kit; TPAR1 ELISA Kit; C-X-C motif chemokine 12 ELISA Kit; Pre-B cell growth-stimulating factor ELISA Kit; PBSF ELISA Kit; Thymic lymphoma cell-stimulating factor ELISA Kit; TLSF ELISA Kit
缩写：CXCL12
Uniprot No.：P40224
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDF-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 87-91 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of mouse SDF-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 99-107 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.0122.1222.0671.91010001.3681.3561.3621.2055000.8120.8410.8270.6702500.4550.4320.4440.2871250.3010.3020.3020.14562.50.2580.2640.2610.10431.250.2010.2030.2020.04500.1560.1580.157 | ||||||
货期：3-5 working days
×
