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Human Breast and kidney expressed chemokine,BRAK ELISA Kit

  • 中文名: 人胸肾表达趋化因子(BRAK/CXCL14)ELISA Kit
货号: IPD-E10025h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人胸肾表达趋化因子(BRAK/CXCL14)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CXCL14含量。CXCL14是重要靶点。其在多种生理和病理过程中发挥作用,在免疫调节、炎症反应等方面背景关键。研究机制上,它主要通过与相应受体结合,调控细胞的迁移、增殖等行为,对其深入研究有助于开发相关疾病的治疗方法。试剂盒检测范围为0.39 ng/ml-25 ng/ml.,灵敏度为0.09 ng/ml.。适用于体外实验中对不同来源样本的BRAK/CXCL14蛋白表达量分析,可满足基础科研领域如免疫学、肿瘤生物学、分子病理学等研究方向的需求,为探索该靶点在细胞信号传导、组织微环境互作中的功能提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    1110031L23Rik ELISA Kit; 1200006I23Rik ELISA Kit; AI414372 ELISA Kit; BMAC ELISA Kit; bolekine ELISA Kit; BRAK ELISA Kit; Breast and kidney ELISA Kit; C-X-C motif chemokine 14 ELISA Kit; C-X-C motif chemokine ligand 14 ELISA Kit; Chaemokine; CXC motif; ligand 14 ELISA Kit; Chemokine (C-X-C motif) ligand 14 ELISA Kit; Chemokine BRAK ELISA Kit; CXC chemokine in breast and kidney ELISA Kit; CXCL14 ELISA Kit; CXL14_HUMAN ELISA Kit; JSC ELISA Kit; Kec ELISA Kit; Kidney-expressed chemokine CXC ELISA Kit; KS1 ELISA Kit; MGC10687 ELISA Kit; MGC124510 ELISA Kit; MGC90667 ELISA Kit; MIP 2 gamma ELISA Kit; MIP-2G ELISA Kit; MIP2G ELISA Kit; MIP2gamma ELISA Kit; NJAC ELISA Kit; PRO273 ELISA Kit; PSEC0212 ELISA Kit; Scyb14 ELISA Kit; Small Inducible Cytokine B14 ELISA Kit; Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK) ELISA Kit; Small Inducible Cytokine subfamily B; member 14 ELISA Kit; Small-inducible cytokine B14 ELISA Kit; Tumor suppressing chemokine ELISA Kit; UNQ240 ELISA Kit
  • 缩写:
    CXCL14
  • Uniprot No.:
    O95715
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.39 ng/ml-25 ng/ml.
  • 灵敏度:
    0.09 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human BRAK/CXCL14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    88-100


    1:2

    Average %

    95


    Range %

    90-101


    1:4

    Average %

    98


    Range %

    92-105


    1:8

    Average %

    93


    Range %

    86-99


  • 回收率:

    The recovery of human BRAK/CXCL14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    97

    90-110


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    25

    3.056

    2.934

    2.995

    2.828


    12.5

    1.622

    1.667

    1.644

    1.477


    6.25

    1.055

    1.028

    1.041

    0.874


    3.12

    0.656

    0.629

    0.643

    0.475


    1.56

    0.454

    0.471

    0.462

    0.295


    0.78

    0.247

    0.253

    0.250

    0.083


    0.39

    0.191

    0.184

    0.187

    0.020


    0

    0.170

    0.165

    0.167




  • 货期:
    3-5 working days

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